乌头类药材及中毒案例未知品中酯型生物碱的检出及测定

Checking and measuring double-ester alkaloids in aconites medicinal materials and in unknowing materials poisoning cases

目的 :建立乌头类主要毒性成分新乌头碱、乌头碱、次乌头碱的检出方法和含量测定方法。方法 :以 TL C法和 HPL C法双重鉴定各药材及未知品中酯型生物碱的存在并测定其含量。结果 :TLC法能有效检出新乌头碱、乌头碱和次乌头碱 ,HPLC法可进一步确证各酯型生物碱的存在并测定 TL C1/5 0 0点样量的乌头碱含量。结论 :该方法操作简单、快速、准确 。

PURPOSE: Establish the methods to detect and measure new aconitine, aconitine and hypoaconitine, which are the mostly toxic components of aconites. METHOD: The existance and contents of double ester alkaloids in every medicinal materials and unknowing materials were carried out by TLC and HPLC. CONSEQUENCE: TLC was good to separate new aconitine, aconitine and hypoaconitine. HPLC can detect the existane of apiece double ester alkaloids and can measure 1/500 of the quantity by TLC. Conclusion: checking and measuring by TLC and HPLC are simple rapid and accurate method for the assay of aconites medicine materials.