摘要
目的 :建立血清中苯巴比妥 (PB)、苯妥英 (PT)、卡马西平 (CBZ)、氯硝基安定 (CNZP)浓度测定的高效液相色谱法。方法 :以 PB、PT互为内标 ,ODS-Hypersil柱为分析柱 ,甲醇 -水 -β-环糊精 (4 5∶ 5 5∶ 0 .0 1)为流动相 ;流速为 0 .8ml/min,紫外检测波长 2 5 4nm。结果 :PB、PT、CBZ、CNZP分离良好 ,最低检测浓度分别为 0 .2 5、0 .5、0 .0 5、0 .0 15 μg/ml;线性范围分别为 2 .5~40、2 .5~ 40、1.2 5~ 2 0、0 .0 2~ 0 .3 2 μg/ml;相对回收率分别为 97.8%、10 2 .1%、10 2 .3 %、10 3 .3 % ;日内、日间 RSD均小于10 %。结论 :该法操作简便 ,结果可靠 。
AIM: to establish an assay method for the determination of phenobarbital(PB), phenytoin(PT), carbamazepine(CBZ) and clonazepam (CNZP) in serum by high performance liquid chromatography (HPLC). METHODS: Internal standards were phenobarbital and phenytoin. The analytical column was ODS Hypersil column. The mobile phase consisted of methanol water β cyclodextrin (45∶55∶0.01) and the flow rate was 0.8ml/min. The UV wavelength was 254nm. RESULTS: PB, PT, CBZ and CNZP were separated well. The lowest detection concentration of PB, PT, CBZ and CNZP were as following: 0.25, 0.5, 0.05 and 0.015 μg/ml; The linear relation was in the range of 2.5~40, 2.5~40, 1.25~20 and 0.02~0.32 μg/ml. Relative recovery rate were 97.8%, 102.1%, 102.3% and 103.3% respectively. The relative standard deviation (RSD) of within day and between day were all less than 10%. CONCLUSION: The method is found to be sensitive with constant result. The experiment will supply reference methods for the determination of PB, PT, CBZ and CNZP in serum.
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2002年第2期155-156,共2页
Chinese Journal of Modern Applied Pharmacy