米帕明和丁螺环酮抗隔离小鼠攻击行为的药理作用

Anti-aggressive action of imipramine and buspirone in isolated mice

目的 研究米帕明和丁螺环酮对小鼠探究行为、自主活动以及隔离攻击行为的影响。方法 雄性昆明种小白鼠隔离饲养 38～ 45天 ,建立隔离小鼠攻击模型。急性腹腔注射生理盐水或米帕明或丁螺环酮 ,30min后测定小鼠的攻击行为。采用XZC 4A自主活动测定仪 ,测定群居小鼠的探究行为和自主活动性。结果 (1)米帕明各剂量组 (0mg/kg ,2 5mg/kg ,5 0mg/kg ,10 0mg/kg)攻击行为的潜伏期 ( x±s)分别为 (5 2± 2 8)s ,(6 7± 47)s ,(132± 87)s ,(2 2 8± 94)s;呈剂量依赖性延长隔离小鼠攻击行为的潜伏期 ,拮抗其攻击行为。然而 ,同等剂量的米帕明对群居小鼠的自主活动和探究行为无明显影响。 (2 )丁螺环酮 2 5mg/kg,5 0mg/kg ,10 0mg/kg剂量组的攻击行为潜伏期 ( x±s)分别为(2 15± 74)s,(134± 10 3)s和 (30 0± 0 )s。与生理盐水组 (18± 7)s相比较 ,差异有显著性 (P <0 0 5 ) ,可明显抑制隔离小鼠的攻击行为。给小鼠腹腔注射相同剂量丁螺环酮 ,呈剂量依赖性减少群居小鼠的自主活动和探究行为。结论 米帕明和丁螺环酮均具有抗隔离小鼠攻击行为的药理活性。

Objective To study the effects of imipramine and buspirone on spontaneous motor activity, explorative behavior and isolation-induced aggressiveness in mice. Methods Male Kunming mice were isolated for 38～45 days as an animal model of isolation-induced aggression. The isolated mice and group-housed mice were intraperitoneally (IP) administrated with normal saline or different doses of imipramine or buspirone. The aggressiveness of isolated mice was measured 30 minutes after the administration. The exploration and spontaneous motor activity in group-housed mice was measured with the apparatus of locomotion for mice (XZC-4A). Results (1)The latencies (±s) to attacking in imipramine (0, 2.5, 5.0 or 10.0 mg/kg) groups were (52±28), (67± 47),(132±87) and (228±94) seconds respectively, which showed imipramine could dose-dependently increase the latency to attacking and antagonize aggressive behaviors in isolated mice. However, imipramine of same doses did not affect spontaneous motor activity and explorative behavior in group-housed mice. (2)The latencies to attacking in buspirone (2.5, 5.0 and 10.0 mg/kg) group were (215±74), (134±103) and (300±0) seconds respectively, which were significantly different from normal saline group (18±7)seconds and obviously inhibited aggression in isolated mice. Buspirone could also dose-dependently decrease spontaneous motor activity and explorative behavior in group-housed mice. Conclusion Both imipramine and buspirone are of antagonization against aggressive behaviors induced by socially isolation in mice.