期刊文献+

气压致离体中枢神经细胞损伤模型 被引量:6

Injury model of central neuron by air pressure in vitro
下载PDF
导出
摘要 目的 建立一种空气高压力方法导致体外培养神经细胞的损伤模型 ,用于中枢神经系统各个细胞群及单个细胞在损伤中反应的研究 .方法 在空气高压的状态下致神经细胞损伤 ,并利用碘化丙啶 (PI)荧光探针不能穿过正常的细胞膜、当细胞膜损伤后其通过细胞膜并与细胞核的 DNA结合、在荧光显微镜下显红色及乳酸脱氢酶 (L DH)的测定来检测细胞损伤 .结果 当空气压力增加到 1.0 MPa时 ,细胞损伤较对照组明显增加 ,压力增加到 1.5 MPa时 ,得到较为理想的损伤模型 .结论 本神经细胞损伤模型适合神经细胞损伤的病理。 AIM To establish a method of cultured neuron injury models by high air pressure for the study of injured cell group or single cell in the central nervous system (CNS). METHODS Neuron injuring model was produced by using high air pressure and assessed by nuclear uptake of the fluorescent dye propidium iodide (PI), which is excluded from cell with intact membranes however the dye penetrates injured cells and binds to DNA in the nuclei which then fluo resces red. Lactate dehydrogenase (LDH) enzyme was measured spectrophotometrically. RESULTS High air pressure of 1.0 MPa caused neuron injury and got the ideal injuring model by the air pressure of 1.5 MPa. CONCLUSION This model is appropriate for studying the biochemistry and pathophysiology of neuron injury.
出处 《第四军医大学学报》 北大核心 2002年第5期423-425,共3页 Journal of the Fourth Military Medical University
关键词 中枢神经 细胞损伤模型 空气压力 碘化丙啶 乳酸脱氢酶 cell injuring model air pressure propidium iodide lactate dehydrogenase
  • 相关文献

参考文献15

  • 1[1]Tavalin SJ, Ellis EF, Satin LS. Inhibition of the electrogenic Na pump underlies delayed depolarization of cortical neurons after mechanical injury or glutamate [J]. J Neurophysiol, 1997; 77(2): 632-638.
  • 2[2]Bleiberg J, Kane RL, Reeves DL, Garmoe WS, Halpern E. Factor analysis of computerized and traditional tests used in mild brain injury research [J]. Clin Neuropsychol, 2000;14(3):287-294.
  • 3[3]Lee VM, Thibault LE. An in vitro model of traumatic neuronal injury: Loading rate-dependent changes in acute cytosolic calcium and lactate dehydrogenase release [J]. J Neurotrauma, 1997;14(6): 355-368.
  • 4[4]Movsesyan VA, O'Leary DM, Fan L, Bao W, Mullins PG, Knoblach SM, Faden AI. mGluR5 antagonists 2-methyl-6-(pheny-lethynyl)-pyridine and (E)-2-methyl-6-(2-phenylethenyl)-pyridine reduce traumatic neuronal injury in vitro and in vivo by antagonizing N-methyl-D-aspartate receptors [J]. J Pharmacol Exp Ther, 2001;296(1):41-47.
  • 5[5]Ostrow LW, Langan TJ, Sachs F. Stretch-induced endothelin-1 production by astrocytes [J]. J Cardiovasc Pharmacol, 2000;36(5 Suppl 1):S274-S277.
  • 6[6]Nakayama Y, Aoki Y. Mechanism responsible for the formation of focal swellings on injured neuronal processes using a novel in vitro model of axonal injury [J]. Forensic Sci Int, 2000;113(1-3):245-249.
  • 7[7]Situ ZQ, Wu JZ. Xibao Peiyang (Cell culture) [M]. Xi'an: Shijie Tushu Chuban Gongsi (World Publishing Corporation), 1996:121-125.
  • 8[8]Ellis EF, Mckinney JS, Willoughby KA, Liang S, Povlishock JT. A new model for rapid stretch-induced injury of cells in culture: Characterization of the model using astrocytes [J]. J Neurotrauma, 1995;12(3):325-339.
  • 9[9]Jones KH, Senft AJ. An improved method to determine cell viability by simultaneous staining with fluorescein diacetate-propi-dium iodide [J]. J Histochem Cytochem, 1985;33:77-79.
  • 10[10]Murphy EJ, Horrocks LA. A model for compression trauma: Pressure-induced injury in cell cultures [J]. J Neurotrauma, 1993;10(4):431-444.

同被引文献59

引证文献6

二级引证文献17

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部