气压致离体中枢神经细胞损伤模型

Injury model of central neuron by air pressure in vitro

目的 建立一种空气高压力方法导致体外培养神经细胞的损伤模型 ,用于中枢神经系统各个细胞群及单个细胞在损伤中反应的研究 .方法 在空气高压的状态下致神经细胞损伤 ,并利用碘化丙啶 (PI)荧光探针不能穿过正常的细胞膜、当细胞膜损伤后其通过细胞膜并与细胞核的 DNA结合、在荧光显微镜下显红色及乳酸脱氢酶 (L DH)的测定来检测细胞损伤 .结果 当空气压力增加到 1.0 MPa时 ,细胞损伤较对照组明显增加 ,压力增加到 1.5 MPa时 ,得到较为理想的损伤模型 .结论 本神经细胞损伤模型适合神经细胞损伤的病理。

AIM To establish a method of cultured neuron injury models by high air pressure for the study of injured cell group or single cell in the central nervous system (CNS). METHODS Neuron injuring model was produced by using high air pressure and assessed by nuclear uptake of the fluorescent dye propidium iodide (PI), which is excluded from cell with intact membranes however the dye penetrates injured cells and binds to DNA in the nuclei which then fluo resces red. Lactate dehydrogenase (LDH) enzyme was measured spectrophotometrically. RESULTS High air pressure of 1.0 MPa caused neuron injury and got the ideal injuring model by the air pressure of 1.5 MPa. CONCLUSION This model is appropriate for studying the biochemistry and pathophysiology of neuron injury.