心房利钠肽基因多态性与中国汉族2型糖尿病患者蛋白尿关系的研究

A study on the association between at rial natriuretic peptide(ANP)gene polymorphism and proteinuria i n type 2diabetes mellitus.

目的:探讨心房利钠肽(ANP)基因多态性与中国汉族2型糖尿病患者蛋白尿之间的关系。方法:应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术对2型糖尿病患者中无蛋白尿的患者50例、微量蛋白尿患者43例、大量蛋白尿患者40例以及正常对照组42名进行ANP基因C/T多态性研究。结果:研究对象具有限制性内切酶BstXI酶切位点的T等位基因频率显著高于白种人;对照组及糖尿病各组之间T及C等位基因和基因型频率均无统计学差异。结论:ANP基因C/T多态性与中国汉族2型糖尿病患者蛋白尿无相关性,可能不是中国汉族2型糖尿病肾病的主要遗传标志。

Objective:To explore the association between a trial natriuretic peptide(ANP)gene polymorphism and proteinuria of type 2diabetes mellitus(DM)in Chinese.Methods :ANP gene C /T polymorphism was studie d by polymerase chain reaction and restriction fragment length polymo rphism(PCR-RFLP)analysis.The samples were collecte d from 50patients of normalbuminuric,43patients of microalbuminuric,40patients of macroalbuminuric wit h type 2DM and 42healthy individuals as controls.Results:In all the examinees,the frequency of the BstXI allele T wa s much higher than in white men.There was no difference in genotype or allele frequencies among any groups.Conclusion:Our study showes the lack of association between ANP gene C /T polymorphis m and proteinuria in type 2DM of Chinese.The polymorphism may not be a major genetic marker of diabetic nephropathy.