侵袭性白念珠菌小鼠感染模型的建立与评估

Establishment and evaluation of invasive Candida albicans infection model in mice

目的建立侵袭性白念珠菌小鼠感染模型。方法体外通过菌丝生长实验评价白念珠菌的毒力大小,通过尾静脉注射白念珠菌菌悬液感染小鼠后,观察小鼠生存时间,评价白念珠菌体内毒力大小;以感染后7 d内小鼠100%死亡为标准,建立了侵袭性白念珠菌小鼠感染模型,对感染小鼠进行氟康唑治疗效果评价。结果白念珠菌体外毒力与体内毒力一致,4种培养基中菌丝均生长良好的菌株,体内感染小鼠后,小鼠生存率也最低;感染模型中,氟康唑对敏感白念珠菌的治疗效果强于对氟康唑耐药白念珠菌(P<0.05)。结论建立的侵袭性白念珠菌感染模型可用于药物体内抗白念珠菌活性的筛选。

Objective To establish an invasive Candida albicans infection model in mice. Methods The virulence of Candida albicans in vitro was evaluated by means of mycelial growth experiment,while that in vivo was evaluated through the mouse infection model;an aliquot of culture containing Candida albicans with 5 ＆#215; 105 CFU was injected into each mouse through the tail vein and the survival time of the infected mice was observed. 100% death of the mice within 7 days of infection was used as the successful establishment of the infection model;fluconazole was applied to the infected mice and the effect on the infection of Candida albicans was evaluated as well. Results Candida albicans had the same virulence in vitro and in vivo;strains were well inducted in the 4 hyphal induction media;the survival rate of the infected mice was very low;fluconazole was more effective on Candida albicans sensitive to it than on those resistant to it（ P ﹤ 0. 05）. Conclusions Invasive Candida albicans infection model in mice is applicable for drug screening of anti-Candida albicans activity in vivo.