磷酸钙微晶玻璃植入兔骨缺损区形态及组织学观察

A histological study on experimental implantation of crystalline glass containing β-TCP in rabbit's mandible

目的 :观察磷酸钙微晶玻璃在植入兔颌骨后的组织学变化 ,探索磷酸钙陶瓷的生物降解机制。方法 :可吸收性微晶玻璃 (ACG)、烧结型可吸收性微晶玻璃粉 (ACGP)植入兔颌骨人工缺损区后观察X线片、横截面积测量、光镜、电镜及能谱分析的变化。结果 :ACG、ACGP及 β TCP随植入时间的增加 ,材料横截面积逐渐缩小 ;X线能谱分析提示材料界面区钙、磷、硫 3种元素含量都逐渐接近正常骨水平。结论 :磷酸钙微晶玻璃具有良好的生物相容性 。

?Objective:To study the inductive effect of absorbable crystalline glass (ACG) and absorbable crystalline glass powder(ACGP) , in osteogenesis.Methods:Samples of ACG,ACGP and β TCP in the size of 10 mm×3 mm×2.5 mm were prepared and implanted subperiosteally into the mandible deffects in 48 rabbits .Specimens obtained 2,4,12,and 24 weeks after implantation were observed with X ray film,measurement of area of crosssection energy dispersive X ray analysis(EDX),light and scanning electron microscopes.Results:Bone formation was found in 12 weeks and increased in 24 weeks in all implannts.The degradation (%) of ACG,ACGP and β TCP in 24 weeks were 33,80 and 40 respectively.24 weeks after implantation the Ca ++ content (% of wt)in ACG,ACGP,β TCP and rabbit mandible were 20.54,20.29,19.96 and 19.71;the P ++ content (% of wt)10.54,10.51,10.72 and 10.96,respectively.Conclusion:ACG and ACGP are degradable,biocompatible and osteoinductive.