β-胡萝卜素对病毒转化细胞的影响

Effect of beta-carotene on growth of 293 cells and Raji cells in vitro

目的 探讨β-胡萝卜素 (β- C)对病毒转化细胞的抑制作用 .方法 体外培养人腺病毒转化的 2 93细胞和 EB病毒转化的 Raji细胞 ,培养时添加 0 ,10 ,5 0和 10 0 μmol· L- 1的β- C作用 2 4,48和 72 h后 ,进行细胞计数 ,测定细胞生长抑制率 ;EL ISA-结晶紫方法测定 A值 ,计算细胞存活量 ;采用快速 CPE(细胞病变 )方法测定复制缺陷型腺病毒在 2 93细胞上的病毒滴度 .结果 10～ 10 0μmol· L- 1 的β- C对 2 93细胞和 Raji细胞均有抑制作用 ,细胞存活量减少 ,抑制率分别是15 .6 % ,10 .1% (10 μm ol· L- 1 ) ;19.7% ,2 2 .4% (5 0 μmol· L- 1 )和 33.6 % ,2 9.3% (10 0μmol· L- 1 ) ,P<0 .0 5 .快速CPE结果显示加入 β- C后 ,复制缺陷型腺病毒在其辅助细胞 -2 93细胞上的复制增殖能力明显下降 ,病毒滴度降低 ,空斑形成单位 (× 10 9pfu· L- 1 )分别为 98± 41(10μmol· L- 1 ) ,75± 2 9(5 0 μmol· L- 1 )和 6 3± 31(10 0 μmol· L- 1 ) ,与对照组 185±2 5 (0 μmol· L- 1 )相比 ,P<0 .0 5 .结论 β- C对病毒转化的2 93细胞和 Raji细胞的生长增殖具有明显的抑制作用 ,对 2 93细胞内整合的腺病毒早期基因

AIM To investigate the inhibitory effect of beta-carotene on virus-transformed cells. METHODS Human adenovirus transforming 293 cells and EB virus transforming Raji cells were cultured in medium containing 0, 10, 50 and 100 μmol·L -1 beta-carotene for 24, 48 and 72 h. Cells were counted and inhibition rate of growth was detected. ELISA-Crystal violet method was used to detect survival cells. Rapid CPE (cell pathology effect) method was applied to measure the titer of replicate-defect adenovirus in 293 cells. RESULTS Beta-carotene at concentrations of 10~100 μmol·L -1 inhibited significantly the proliferation of 293 cells and Raji cells (P<0.05). Inhibition percentages were 15.6%, 10.1% (10 μmol·L -1), 19.7%, 22.4% (50 μmol·L -1) and 33.6%, 29.3% (100 μmol·L -1), respectively. The survival cells decreased obviously .The titer of replicate-defect adenovirus in 293 cells dropped significantly (P< 0.05). Plaque formation unit (×10 9 pfu·L -1) were 98±41 (10 μmol·L -1), 75±29(50 μmol·L -1) and 63±31 (100 μmol·L -1), respectively. CONCLUSION Beta-carotene can inhibit proliferation of 293 cells and Raji cells and down-regulate expression of virus gene in 293 cells significantly.