普乐可复对大鼠小肠移植排斥反应的免疫抑制作用

Immunosuppression Effect of FK506 on the Transplant Rejection in small bowel transplantation in rats

目的 观察大鼠小肠移植术后应用普乐可复(FK5 0 6 )对小肠移植排斥反应的免疫抑制作用 .方法 选用近交系大鼠 F34 4/ N(RT 1 )和 Wistar大鼠进行全小肠异位移植 ,实验分 4组 .第 1组 ,异基因移植组 F34 4/ N- Wistar;第2组 ,小剂量普乐可复治疗组 F34 4/ N- Wistar+FK5 0 6 (0 .2mg· kg- 1 · d- 1 ,0～ 14d,im ) ;第 3组 ,普乐可复治疗组F34 4/ N- Wistar+FK5 0 6 (0 .5 m g· kg- 1· d- 1 ,0～ 14d,im ) ;第 4组 ,同基因移植组 (F34 4/ N- F34 4/ N ) .观察大鼠的一般状况及存活时间并于术后第 3,5 ,7日取移植小肠标本进行病理学检查和比较 .结果 第 1组大鼠存活时间与其他3组有显著差异 (P<0 .0 1) ,第 1组大鼠在术后第 3,5 ,7日病理学检查移植肠出现排斥反应 ,而第 2 ,3,4组无明显排斥 ,可长期存活 .结论 普乐可复能明显抑制小肠移植急性排斥反应的产生 。

AIM To observe the immunosuppression of FK506 on the transplant rejection after small bowel transplantation in rats. METHODS Heterotopic small bowel transplants was carried out between inbred F344/N and Wistar rats. The recipients were divided into four groups, group Ⅰ, F344/N-wistar; group Ⅱ, F344/N-wistar+FK506 (0.2 mg·kg -1·d -1, 0~14 d, im); group Ⅲ, F344/N-wistar+FK506 (0.5 mg·kg -1·d -1, 0~14 d, im); groupIV, F344/N-F344/N. Post transplant clinical course and survial duration were recorded and the grafts were harvested on post-operative day 3, 5, 7. All samples were examined pathologically and compared with each other. RESULTS The survial duration of group Ⅰ was significantly different from that of the other tree groups. The pathological examination of the transplantated bowel specimen from group Ⅰ showed that the transplant rejection on post-operative day 3, 5 and 7 while none of group Ⅱ, Ⅲ and Ⅳ had pathological evidence of transplant rejection. CONCLUSION FK506 could obviously suppress the transplant rejection in small bowel transplantation in rats and significantly prolong the survial duration.