肝癌生长、转移中的微血管生成机制

Investigation of the mechanism of angiogenesis in growth and metastasis of HCC

目的 研究微血管生成在肝癌、肝硬化、正常肝间的差异及其与肝癌生长、转移、病理特点的关系 .方法 用 因子抗体和 APA试剂盒对 45例肝癌、2 1例肝硬化和 8例正常肝的标本行免疫组织化学染色 ,然后在 2 0 0 (× )显微镜下计数每一视野下的微血管数目 .结果 肝癌、肝硬化、正常肝组织的微血管数目分别是 6 7.4± 2 3.8,14.4± 5 .8和 3.5±2 .0 ,经统计学分析 ,三者相差显著 (P<0 .0 1) ,微血管数与肝癌的转移、包膜形成相关 ,而与肝癌的大小、分级无关 .结论 微血管生成在肝癌的转移。

AIM To investigate the difference and the mechanism of angiogenesis among hepatocellular carcinoma (HCC), cirrhosis and normal liver. METHODS With antibody of Ⅷ factor and APA kit, paraffin-embedded slides of 45 HCC, 21 cirrhosis and 8 normal liver were stainned, after which, microvessel counting was performed. RESULTS The microvessel density (MVD) of HCC, cirrhosis and normal liver was 67.4±23.8, 14.4±5.8 and 3.5±2.0 respectively. The difference was significant statistically. The MVD was correlated with the metastasis and capsule formation of HCC. CONCLUSION Angiogenesis may play an important role in the metastasis and capsule formation of HCC.