输血传播病毒TTV致病性的探讨

Pilot study on pathogenicity of transfusion transmitted virus

目的 通过病例对比 ,进一步研究 TTV是否有致病性 ;对 1例西安地区非甲 -非戊型肝炎但血清 TTV阳性患者套式 PCR终产物纯化测序 ,在验证我们 TTV DNA检测正确性的同时初步研究西安地区 TTV流行株部分分子生物学特点 .方法 在 TTV ORF1区参照文献 [1]设计一套套式 PCR引物 ,并建立稳定的套式 PCR检测法 ,对西安地区 119例非甲 -非戊型肝炎患者及 16 2例健康有偿献血员进行 TTV对比检测和统计学处理 ;对 1例患者套式 PCR终产物纯化测序 ,用 DNAsis软件在计算机上与中国 1株做同源性比较阳性 .结果 119例非甲 -非戊型肝炎患者中 37例 TTV DNA阳性 ,阳性率 31.1% ;16 2例健康有偿献血员中 TTV DNA阳性 30例 ,阳性率 18.5 % ;两组有明显的统计学差异 (P<0 .0 5 ,χ2 =5 .2 5 1) .对 1例非甲 -非戊型肝炎患者血清 TTV套式 PCR终产物纯化直接测序 ,测得 143bp序列 ,经计算机比较 ,其与中国 1株同源性为 6 5 .0 % ,差异率为 35 .0 % (>30 .0 % ) .其中6 2 bp序列同源性高达 75 .8% .结论 TTV可能有致病性 ;西安地区可能存在新的

AIM To study pathogenicity of transfusion transmitted virus (TTV) and sequence part of TTV DNA in Xi'an. To compare partial sequence of TTV in Xi'an with DBH1, which were reported by WANG Hai-tao who is a professor of the Academy of Military Medical Sciences, in computer with software of DNAsis to find their homogeneity .METHODS A nested polymerase chain reaction (nested-PCR) assay with two pair of primers from ORF1 of TTV genome was established to detect TTV DNA in serum samples from 119 cases of non-A to non-E hepatitis patients and 162 cases of healthy blood donors. Their ALT and AST were detected in their serum too.RESULTS TTV DNA were detected in serum from 37 of 119 (31.1%) non-A to non-E hepatitis patients and 30 of 162 (18.5%) healthy blood donors. These two groups had statistical difference (χ 2= 5.251, P<0.05). 3 of 37 cases of non-A to non-E hepatitis patients with TTV DNA positive were cirrhosis, the other 30 cases of non-A to non-E hepatitis patients with TTV DNA positive were acute hepatitis. The partial sequence of TTV (143 bp) in Xi'an was sequenced and its homology with BDH1 (Gen Bank AF116842) was 65.0% after comparason with computer. CONCLUSION TTV may have pathogenicity, it can cause acute and chronic hepatitis. The results of comparason of the partial sequence of TTV in Xi'an with BDH1 suggest that there may be new genicity of TTV in Xi'an.