摘要
为了给马铃薯(Solanum tuberosum L.)抗寒品种选育提供基因资源,采用测定叶片渗透率、SOD酶活性以及离体叶片低温胁迫方法对地方品种普安紫洋芋和费乌瑞它自然及辐射变异株系的抗寒性进行鉴定。结果显示:从处理组间渗透率与SOD来看,普安紫洋芋和费乌瑞它变异株系不同温度处理组的渗透率与SOD检测结果一致,均是常温对照组(CK)>0℃(或-3℃)处理组>4℃处理组;各处理单株之间渗透率与SOD差异较大。从离体叶片低温胁迫鉴定来看,当最低处理温度为-6℃时,费乌瑞它自然变异抗寒株系Ⅲ-25-5的存活率为85.7%,其CK的存活率为57.1%;当最低处理温度为-7℃时,费乌瑞它辐射变异株系3-23X的叶片存活率为42.9%,其CK的存活率为7.1%。普安紫洋芋和费乌瑞它变异株系不同单株间抗寒能力表现出明显差异,在其中进行单株抗寒性的选择有效果。
In order to provide genetic resources of cold resistance for breeding potato, the cold resistance ability was identified by determina- tion leaf.permeability, SOD activity and low temperature stress using Puan purple potato and mutation lines of Favorita naturally and radia- tion. The results showed that the permeability determination of Puan purple potato and Favorita mutation lines were similar( normal tempera- ture group (CK) 〉 0 ℃ or 3 ℃ group 〉 4 ℃ ). The differences of individual permeability were obvious. The results of SOD enzyme activi- ty detection were consistent with the results of permeability test. The leaf survival rate of Favorita naturally mutation lines 111-25-5 was 85.7 % for and its CK was 57.1% when the minimum processing temperature was -6 ℃. When the minimum processing temperature was -7 ℃, the leaf survival rate of Favorita radiation mutation lines 3-23X was 42.9 % and its CK was 7.1%. The plants cold resistance ability were obvious different for Puan purple potato and Favorita mutation lines. The plant selection of cold resistance ability was effective.
出处
《西南农业学报》
CSCD
北大核心
2014年第3期991-995,共5页
Southwest China Journal of Agricultural Sciences
基金
贵州省科技攻关项目"马铃薯诱变新品系选育及变异基因的分子检测研究"[黔科合NY字(2011)3021]
贵州省农委动植物专项"表型及分子标记鉴定筛选马铃薯诱变新材料的研究"[黔农育专字(2011)029]
贵州省科技计划项目"贵州类育种及产业化研发平台建设[黔科创能院所(2010)4006]
贵州省科技计划项目"贵州薯类育种及产业化研发平台建设"[黔科创能院所(2010)4006-(07
11)]
贵州省农业科学院专项"转基因马铃薯的鉴定和安全性评估"[黔农科院专项(2009)002]
关键词
马铃薯
抗寒性
渗透率
SOD
低温胁迫
Potato
Cold resistance
Permeability
SOD detection
Low temperature stress