新洋葱伯克霍尔德菌NSM-05产抗菌物质的培养条件

Cultural conditions optimizing for the productivity of antagonistic metabolites by Burkholeria cenocepacia strain NSM-05

为了提高拮抗细菌新洋葱伯克霍尔德菌NSM-05产生抗菌物质的能力,分别采用单因素、正交试验和实验条件的优化等方法对NSM-05菌株产生抗菌物质的最佳培养条件进行研究。结果表明,NSM-05菌株在产生抗菌物质最多的培养条件为:玉米粉质量分数为0.45%,蛋白胨为1.0%,NaCl为0.75%,MgSO4为0.25%,pH为6.5,培养温度25℃,通气量≥50%,摇床速度200 r/min,培养时间为48 h。

In order to obtain more antagonistic metabolites of Burkholeria cenocepacia NSM-05, the optimum culturing conditions were screened by using the single factors tests, orthogonal tests and optimized fermentation. The results showed that the optimal fermentation conditions for production of antagonistic metabolites were cornmeal 0.45%, peptone 1%, NaC1 7.5%, MgS04 2.5%, pH 6.5, culturing temperature 25 ℃, ventilatory capacity≥ 50%, vibration speed 200 r/min, culturing time 48 h.