绿液预处理对毛竹化学成分及酶水解糖化的影响

Effects of green liquor pretreatment on chemical composition and enzymatic hydrolysis of moso bamboo

毛竹是重要的木质纤维素类能源作物之一,为提高毛竹的酶水解糖化效率,研究了不同总用碱量(TTA)和温度下,绿液(碳酸钠+硫化钠)预处理对毛竹的化学成分及酶水解的影响。结果表明:随着预处理用碱量增加和温度的提高,木质素的脱除率上升,但同时也加剧了碳水化合物的降解。预处理后的浆料用CellicCTec2复合酶进行酶水解,结果表明随着预处理用碱量和温度的提高,各聚糖得率均有所上升。优化得到绿液预处理毛竹最优条件为:最高温度160℃,用碱量16%,硫化度25%。此条件下的浆料得率为68.3%,脱木质素率为44.6%。所得浆料在纤维素酶用量40μmol/(min·g)下酶水解后,葡聚糖、木聚糖和总糖得率分别为64.9%、48.0%和58.1%。

Moso bamboo is one of the abundant lignocellulosic biomass resources for the production of bioenergy. For improving the efficiency of enzymatic saccharification,the effects of green liquor（ Na2CO3＋ Na2S） pretreatment on the chemical composition and the enzymatic hydrolysis of moso bamboo were investigated at different total titratable alkaline（ TTA） and temperature. The results showed that the increasing of TTA charge and temperature resulted in degradation of polysaccharides increasing. The hydrolysis sugar yield went up with the increase of TTA charge and temperature in the pretreatment. Under optimized conditions（ TTA charge 16%,sulfidity 25%,and temperature 160 ℃）,the pretreated solid recovery was 68. 3% with a lignin removal of 44. 6%. The yields of glucan,xylan and total sugar of green liquor pretreated poplar in enzymatic hydrolysis were 64. 9%,48. 0% and 58. 1%,respectively at cellulase loading of40 μmol /（ min·g）.