富油卵形扁藻自养培养条件的研究

Autotrophic Culture of High-oil Platymonas subcordiformis and Optimization of Growth Conditions

为了提高能源微藻——富油卵形扁藻的生物量,以f/2培养基作为培养基,对营养盐氮(NaNO3)、磷(KH2PO4)、铁(FeCl3)、不同人工海水进行优化,采用单因素和L9(34)正交试验进行优化。结果表明:Fe3+的摩尔浓度为0.08 mmol/L、KH2PO4的质量浓度为11 mg/L、NaNO3的质量浓度为0.3 g/L和人工海水的2号配方适于富油卵形扁藻的生长,在以后的后续试验中均以此培养基配方进行培养。该海藻在优化后的培养基中生长情况良好,稳定期最大干重生物量可达452 mg/L,可作为后续生物柴油生产的原料。

In order to improve energy Platymonas subcordiformis biomass, f/2 medium was chosen as basal medium for optimizing the nutrients such as Fe3＋, P, N and artificial seawater. The cultivating concentrations of the nutrients Fe3., P, N and artificial seawater were optimized using a single factor experiment and L9（3＋） orthogonal design method. Results showed in the autotrophic culture of Platymonas subcordiformis, the optimal concentrations of the nutrients was chelated iron ions 0.08 retool/L, monopotassium phosphate 11 mg/L, sodium nitrate 0.3 g/L and the second chemical ingredients of artificial seawater. High-oil spherical microalgae grew very well in the optimized medium, with the maximum biomass as 452 rag/L, which indicated that high-oil algae may be a raw material of the biodiesel production.