甘蔗多样性微阵列技术(DArT)标记体系的建立

Construction of DArT system in sugarcane

[目的]建立成熟、稳定的甘蔗多样性微阵列技术（Diversity array technology,DArT）标记体系,为甘蔗的遗传多样性分析、遗传图谱构建及标记辅助育种等提供新的分子标记方法.[方法]以7份不同种属的甘蔗材料为样本,优化基因组复杂性降低方法并建立甘蔗DArT标记体系,同时应用该体系分析7份材料的遗传多样性.[结果]获得了基于PstⅠ/TaqⅠ酶切组合的最优基因组复杂性降低方法,利用该方法建立了DArT体系.利用DArT体系分析了7份材料之间遗传进化关系,发现供试7份材料的遗传相似系数为0.497～0.811,平均值为0.569.以遗传相似系数0.546为阀值,可以将供试材料分为三大类,其中第一类的GT29号、拔地拉和割手密GXS85-30均为甘蔗属材料,与预期相一致;第二类为河八王2号和滇蔗茅2号,第三类为芒84-8和斑茅GXA87-36.[结论]建立的甘蔗DArT标记体系具有有效性,其遗传相似性分析结果与材料预期的亲缘关系密切程度相一致.

[Objective]A mature and stable sugarcane DArT （Diversity array technology,DArT） system was constructed to provide a new molecular marker method for genetic diversity analysis,genetic map construction and marker-assisted breeding in sugarcane.[Method]Seven different sugarcane germplasms were used to optimize genomic complexity reduction method,develop DArT system and analyze their genetic diversity.[Result]The optimum genome complexity reduction method was obtained based on the combination of Pst Ⅰ/Taq Ⅰ,and then DArT system was built which was used to analyze genetic evolution relations among the 7 materials.The genetic similarity coefficient of 7 materials ranged from 0.497 to 0.811 with an average of 0.569.At the genetic similarity coefficient value of 0.546,7 materials were divided into 3 groups.In the first group,GT29,Badila and GXS85-30 （Saccharum spontaneum） all belonged to Saccharum genus.Narenga porphyrocoma and Erianthus rockii were clustered into the second group.The third group included Miscanthus floridulus 84-8 and GXA87-36.[Conclusion]Sugarcane DArT system constructed in this study was effective,because the results of genetic similarity analysis using the system was consistent with the predicted genetic relationship of the tested materials.