草菇木质纤维素酶活测定及其对木薯渣生物转化效率的影响

Lignocellulolytic enzyme activities of Volvariella volvacea strains and their effects on biological conversion efficiency of cassava residue

【目的】研究不同草菇菌株在木薯渣栽培基质中的木质纤维素酶活性和生物效率及其相关性,为筛选和培育适宜的草菇菌株及提高木薯渣栽培草菇的效率提供科学依据。【方法】采用木薯渣为主要原料的熟料基质,进行17个草菇菌株的栽培,测定针头期基质内的羧甲基纤维素酶(CMCase)、木聚糖酶(Xylanase)和漆酶(Laccase)活性,统计草菇的生物学效率,并采用SPSS18.0软件进行酶活性与产量的相关分析。【结果】3种木质纤维素酶在不同草菇菌株间存在一定差异,其中羧甲基纤维素酶活性较高的菌株有V9、V26、V10、V112、Vh和V木-2,木聚糖酶活性较高的菌株有V9、V112和Vh,漆酶活性较高的菌株有V9、V112、VL和V木-2。不同草菇菌株在木薯渣栽培基质上的生物学效率存在明显差异,其中V112的生物学效率最高,达29.5%;其次是V9和V木-2,两个菌株的生物学效率均高于20.0%。羧甲基纤维素酶、木聚糖酶和漆酶活性与生物学效率均呈正相关,相关系数(R2)分别为0.2650、0.2545和0.4729。【结论】羧甲基纤维素酶、木聚糖酶和漆酶都是草菇高效转化木薯渣基质的重要影响因子,其中漆酶是关键的限制性因子。17个草菇菌株中,V9、V112和V木-2的生物学转化效率较高,是适宜木薯渣配方基质栽培的草菇菌株。

[Objective]Lignocellulolytic enzyme activities of Volvariella volvacea strains and their effects on biological conversion efficiency of cassava residue were studied in order to provide scientific references for screening and breeding desirable strains of Volvariella volvacea and improving cultivation efficiency of cassava residue on Volvariella volvacea.[Method]17 strains of Volvariella volvacea were cultivated on the cooked substrates using cassava residue as main constitutes.CMCase,Xylase and Laccase activities in the substrate were detected at pin stage.Statistics of biological efficiency were studied.The relationship between enzyme activities and fruit body yields was analyzed using SPSS 18.0 software.[Result]Activities of CM-Case,Xylase and laccase of Volvariella volvacea varied significantly.V9,V26,V10,V112,Vh and V wood-2 had high CM-Case activities.V9,V 112 and Vh had high Xylase activities.V9,V 112,VL and V wood-2 had high laccase activities.Biological efficiency of different strains on cassava residue was significantly different.V 112 had the highest biological efficiency （29.5％） followed by V9 and V wood-2 （above 20.0％）.Activities of CMCase,Xylase and laccase were positively related to biological efficiency and the relation coefficient （R2） was 0.2650,0.2545 and 0.4729,respectively.[Conclusion]CMCase,Xylase and laccase were all important factors affecting the conversation of cassava residue by Volvariella volvacea,and laccase was a key limiting factor.Among the 17 Volvariella volvacea strains,V9,V112 and V wood-2 had the highest biological conversion efficiency,so they were optimal strains for cassava residue substrate cultivation.