摘要
建立了药渣中四环素及其衍生物残留的高效液相色谱检测方法.样品经pH 4.0的EDTA-Mcll-vaine缓冲溶液提取后,用Agilent HC-C18色谱柱,以甲醇：乙腈（1∶2,V/V）,含0.01 mol/L草酸为流动相,梯度洗脱,流速0.8 mL/min,检测波长300nm.结果表明,方法检出限为0.05～0.10 mg/L,在0.1～100.0 mg/L范围内线性关系良好,加标回收率为85.8％～100.5％.该方法简便、快速、准确、选择性好,适用于药渣中四环素及其衍生物的定性定量分析.
A high performance liquid chromatographic method （HPLC） was developed to detect tetracycline and its derivatives in tetracycline waste.The samples were extracted with EDTA-Mcllvaine buffer group（pH 4.0）.Tetracycline and its derivatives were successfully separated on Agilent HC-C18 column using methanol-acetonitrile （1∶2,V/V） containing 0.01 mol/L oxalic acid as mobile phase at a flow rate of 0.8 mL/min and were detected at 300 nm.The results showed that the limit of detection was 0.05～0.10 mg/L.The linear range was from 0.1 mg/L to 100.0 mg/L.The recoveries were from 85.8％ to 100.5％.It was simple,rapid,accurate and selective for detecting tetracycline and its from derivatives in tetracycline waste or feed.
出处
《湖北农业科学》
北大核心
2014年第6期1411-1414,共4页
Hubei Agricultural Sciences
基金
国家自然科学基金项目(21067009)
