摘要
目的 检测异嗜性小鼠白血病病毒感染性DNA(pXeno .inf)转染COS 7细胞后形成病毒颗粒的能力。方法 采用脂质体转染方法 ,将异嗜性小鼠白血病病毒感染性DNA转染至COS 7细胞后 ,用电镜观察该细胞中病毒颗粒的形成情况。结果 在异嗜性小鼠白血病病毒感染性DNA转染后的COS 7细胞胞浆中 ,可以见到少量病毒颗粒的存在 ,并且转染后细胞表现出相应的病理变化。结论 证实异嗜性小鼠白血病病毒感染性DNA在COS
Objective To test the virion forming ability of xenotropic murine leukemia virus(MuLV) DNA in COS 7 cells.Methods COS 7 cells was transfected with the xenotropic MuLV DNA by means of liposomal method.The forming of virion particle in the cells was observed by electromicroscope.Results A few of virion particles could be observed in the COS 7 cells transfected with xenotropic MuLV DNA.Pathological changes could also be found in the transfected cells.Conclusion The infective DNA of xenotropic MuLV can synthesize virion in COS 7 cells.
出处
《哈尔滨医科大学学报》
CAS
2002年第2期121-122,共2页
Journal of Harbin Medical University