异嗜性小鼠白血病病毒感染性DNA转染COS-7细胞后形成病毒颗粒的电镜观察

Electromicroscopy observation for virion forming ability of infective DNA of xenotropic murine leukemia virus

目的 检测异嗜性小鼠白血病病毒感染性DNA(pXeno .inf)转染COS 7细胞后形成病毒颗粒的能力。方法 采用脂质体转染方法 ,将异嗜性小鼠白血病病毒感染性DNA转染至COS 7细胞后 ,用电镜观察该细胞中病毒颗粒的形成情况。结果 在异嗜性小鼠白血病病毒感染性DNA转染后的COS 7细胞胞浆中 ,可以见到少量病毒颗粒的存在 ,并且转染后细胞表现出相应的病理变化。结论 证实异嗜性小鼠白血病病毒感染性DNA在COS

Objective To test the virion forming ability of xenotropic murine leukemia virus(MuLV) DNA in COS 7 cells.Methods COS 7 cells was transfected with the xenotropic MuLV DNA by means of liposomal method.The forming of virion particle in the cells was observed by electromicroscope.Results A few of virion particles could be observed in the COS 7 cells transfected with xenotropic MuLV DNA.Pathological changes could also be found in the transfected cells.Conclusion The infective DNA of xenotropic MuLV can synthesize virion in COS 7 cells.