苦参联合胎肝低分子抑瘤物抗白血病及其机制

Anti-leukemia effect of sophora flavescens combined with the low molecular weight natural tumor suppressor of the human fetal liver and its mechanism

目的 :探讨苦参 (SF)、胎肝低分子抑瘤物 (LMW NTS)联合用药对正常人骨髓粒 巨噬系造血祖细胞(CFU GM )和急性髓性白血病细胞的影响及其作用机制。方法 :采用CFU GM集落培养、细胞液体培养、Wrigh giemsa染色、3H TdR掺入实验、DNA片段凝胶电泳和RT PCR等方法 ,观察SF和LMW NTS联用对白血病的作用。结果 :SF和LMW NTS联用 ,明显提高抑制白血病细胞增殖的能力 ,促进白血病细胞的凋亡 ,诱导白血病细胞中原癌基因c Myc的下调 ,抑制正常造血祖细胞CFU GM的作用明显低于抑制白血病细胞的作用。结论 :联合用药抗白血病的效果 。

Objective To explore the effect of sophora flavescens (SF) combined low molecular weight natural tumor suppressor(LMW NTS) on normal human bone marrow CFU GM and acute myelogenous leukemia cells and its mechanism. Methods CFU GM colony culture, cell culture, Wrigh Giemsa stain, 3H TdR incorporation test, agarose gel electrophoresis of DNA fragment and RT PCR were employed in this research. Results The combination of SF and LMW NTS greatly inhibited the proliferation of acute meylogenous leukemia cells, induced their apoptosis, and down regulated the c Myc expression as compared with SF or LMW NTS alone. Conclusion The anti leukemia effect of the combination of SF and LMW NTS is superior to SF or LMW NTS alone.