^(99)Tc-MDP对类风湿性关节炎患者外周血单核细胞产生细胞因子的影响

Effect of ^(99)Tc-MDP on cytokine production by peripheral blood mononuclear cells of patients with rheumatoid arthritis

目的 :探讨“云克”(99Tc MDP)治疗类风湿性关节炎 (RA)的免疫机制。方法 :采用ELISA双抗体夹心法 ,观察“云克”体外对RA患者外周血单核细胞 (PBMC)产生白介素 1(IL 1)和可溶性白介素 2受体 (sIL 2R)的影响。结果 :“云克”有抑制RA患者IL 1的分泌及细菌脂多糖 (LPS)的促分泌作用 ,并对可溶性白介素 2受体的自发分泌及植物血素 (PHA)诱导分泌均有抑制作用。结论 :“云克”对RA的治疗机制可能与其降低IL 1和sIL

Objective To study the immune mechanism of 99 Tc MDP in the treatment of patients with rheumatoid arthritis (RA). Methods In vitro, 99 Tc MDP was added to the cultures of peripheral blood mononuclear cells (PBMC) derived from healthy subjects and RA patients. The levels of IL 1 and sIL 2R from PBMC affected by 99 Tc MDP were determined with enzyme linked immunosorbert assays (ELISA). Results The IL 1 production by PBMC of RA, either in the medium alone (spontaneously) or in the presence of lipopolysacchride (LPS) stimulation, was suppressed by 99 Tc MDP; and the sIL 2R by PBMC of RA, either in the medium alone (spontaneously) or in the presence of phytohemagglutinin (PHA) induction, was inhibited by 99 Tc MDP. Conclusion 99 Tc MDP acts on PBMC and a possible immune activity by 99 Tc MDP is related to the suppression of IL 1 and sIL 2R in RA patients.