摘要
目的 探讨腺病毒介导的野生型p5 3在前列腺癌基因治疗中的可能性及其机制。 方法 培养前列腺癌细胞系PC3M ,转染腺病毒介导的野生型p5 3,检测其增殖和凋亡水平变化 ,并检测其对bax蛋白及mRNA水平的影响。 结果 腺病毒介导的野生型p5 3可高水平转染入PC3M细胞 ,转染率 95 %。转染后的PC3M细胞增殖受到抑制 ,BrdU吸光度A值 0 .4 96 ,显著低于对照组的 1.4 5 4 ,P <0 .0 0 1;细胞凋亡水平增高 ,凋亡指数 16 .2 5 % ,显著高于对照组的 3.6 0 % ,P <0 .0 0 1。细胞中bax蛋白及mRNA水平皆增高。 结论 腺病毒介导的野生型p5 3可抑制PC3M细胞增殖并促进其细胞凋亡 ,其作用可能是通过促进bax表达而实现的。
Objective To investigate the availability and mechanism of recombinant adenovirus vector expressing wild type p53 in gene therapy of prostate cancer. Methods Prostate cancer cell line PC3M was cultivated and recombinant adenovirus vector expressing wild type p53 was added to the media. Then the levels of proliferation and apoptosis were detected and changes of bax were exa mined . Results AdCMVp53 is effectively transfected to the cells. The transfecting ratio is 95%. In the cells transfected with AdCMVp53, the level of proliferation decreased. Tested by BrdU, the absorbance rate decreased from 1.454 in the control group to 0.496 in the experimental group( P < 0.001 ). The level of apoptosis increased.The apoptosis index increasd from 3.60% to 16.25%( P < 0.001 ). There were also an increase in both protein and mRNA level of bax. Conclusions Recombinant adenovirus vector expressing wild type p53 could inhibit the proliferation of PC3M and promote its apoptosis,the mechanism of which may probably due to its affection to bax.
出处
《中华泌尿外科杂志》
CAS
CSCD
北大核心
2002年第3期184-186,共3页
Chinese Journal of Urology