甲壳素—聚已内酯复合接骨板的实验研究

Experiment Study of Chitin-Polycaprolactone Bone Plate

目的 了解可吸收生物接骨板的降解速度及降解时间 ,并观察邻近组织对可吸收生长接骨板的反应。方法 在实验组中 ,用甲壳素纤维增强聚已内酯 (Polycaprolactone、PCL)复合材料制成的可吸收生物接骨板及螺钉 ,包埋于 2 0只新西兰兔右胫骨中段 ;在对照组中 ,选择 8只新西兰兔 ,于相应部位植入金属钢板。实验组动物于手术后 2、 4、 6、 8、 12周处死。对照组于术后 4、 8周时分别处死。所有实验组的实验动物均行生物吸收率 (BAR)测定 ,并行病理切片观察局部组织对接骨板的反应。结果 PCL制成的生物接骨板及螺钉在动物体内降解随时间的推移而增加 ,但不成线性关系。 4周组吸收率为 4 36 % ,6周组为 7 35 % ,12周组为 12 8%。实验组组织学表现为术后 2周时即有较明显的炎症反应 ,6周组更明显 ,此后逐渐减弱 ,但 12周时仍可见炎性细胞。同时可观察到成骨反应。植入对照组未观察到同样的结果。结论 PCL接骨板在体内可吸收 ,但有部分可引起较明显的无菌性炎症反应 ,同时可诱导成骨反应。

Objective To investigate the reabsorbable bone plates degradation speeds with the time change,and observe the neighboring tissue reaction to the reabsorbable bone plate.Methods In the experiment group,using the reabsorbable bone plates and bolts which were made of chitin & PCL,embedding in the right tibia middle piece of rabbit.The follow-up times were two ,four,six,eight,and 12 weeks after operation.Before putting the animal to death.As for the compare group,the reabsorbable bone plates and bolts were instead of metals bone plates and bolts.The follow-up times were four,eight weeks.Then estimated the biologic absorb rate (BAR),and observed local tissue reaction to the bone plate with slice.Results The reabsorbable bone plates and bolts that were made of Chitin and PCL would degrade more and more with time pass in vivo,but the relationship was on tlinearity.The BAR was 4.36% by the 4 weeks,7.35% by the 6 weeks,12.8% by the 12 weeks.In the experiment group,histology showed that there were obviously inflammation two weeks after operation,and more obviously by the 6 weeks,then weakens by degrees,but inflammation still could be observed.At the same time,osteoanagenesis reaction could be observed.In the compare group,there couldn't observe the same phenomenon.Conclusion The reabsorbable bone plates and bolts that were made of Chitin and PCL could be reabsorb,but it would cause obviously sterile inflammatory reaction in some cases.At the same time,it could induce the osteoanagenesis reaction.