摘要
研究了磷脂酶A2 在水相中水解浓缩磷脂的最佳工艺条件 :底物浓度 10 % ,反应温度5 5℃ ,pH值 8 5 ,Ca2 +浓度 0 2mol/L。在最佳条件下得到产品经HPLC检测 ,PC的转化率为92 7% ,并研究了水解速度与时间的关系。水解得到的溶血磷脂产品的HLB大于 8。
The hydrolysis of lecithin by phospholipase A 2 in water was studied, then the optimum conditions of reaction was investigated by the orthogonal experiment, which were substrate concentration 10 percent, reaction temperature 55℃ pH 8.5, Ca 2+ concentration 0.2 mol/l . The convertion of phosphatidycholine was 92.7% under this condition determined by the HPLC. The relationship of rate of hydrolysis and reaction time was also examined. The HLB of lysophospholipids product was over 8.
出处
《中国油脂》
CAS
CSCD
北大核心
2002年第2期83-86,共4页
China Oils and Fats
