L-谷氨酰胺高产菌株的诱变育种

Mutation Breeding of L-Glutamine-Producing Corynebacterium Strain

采用谷氨酸棒杆菌S9114为出发菌株,经γ-射线和硫酸二乙酯诱变处理,定向选育出一株生产菌SH44。在适宜的条件下积累谷氨酰胺平均为41.1mg/ml,最大达41.5mg/ml。

Original strain,Glutamic acid Corynebacterium S9114,was mutated by γ-radiation and DES.Then a strain named WW-42,producing Glutamine was obtained.Under optimum conditions, WW-44 was able to accumulate an average of 28.5mg/ml and the highest of 31.2mg/ml of L-Glutamine.