沙棘果水溶性多糖的分离纯化、组分分析及抗氧化活性的研究

Study on Isolation,Purification,Analysis of the Components and Anti-Oxidation Activity of thePolysaccharides of the Fruits of Hippophae rhamnoides

用热水提取沙棘果多糖,乙醇沉淀,并用正交实验确定最佳条件,Sevage法去蛋白,酸性乙醇分级沉淀,分出A、B、C及D四个组分,其中C用DEAE柱层析进一步纯化出C1和C2两个级分,SephadexG-75及聚丙烯酰胺凝胶电泳证明C1为单一组分,C2为两种多糖混合组分,经薄层层析分析,C1中含葡萄糖。C2的主要级分中含木糖和一未知组分。抗氧化实验表明沙棘多糖具有抗氧化活性,且抗氧化作用随浓度增加而加大。

The polysaccharides of Hippophae rhamnoides were isolated in hot water,and precipitated with perpendicular experiments.The protein in polysaccharides was removed by Sevage way.The polysaccharides were graded to A.B.C and D by acidic ethanol.C was further purified to C1 and C2 with DEAE column chromatography.Sephadex G-75 column chromatography and polyacrylamide gel electrophoresis provde that C1 was one polysaccharide and C2 was two polysaccharides.Their components were analyzed with thin-layer chromatography and the results were that there was glucose in C1 and there were wylose and other unknown monosaccharide in the main component of C2.The anti-oxidation experiments indicated that the polysaccharides of Hippophae rhamnoides had anti-oxidation activty and this activity was increased with the inereased concentration of the polysaccharides.