人参皂甙激活恶性胸水中的TILs及其与吉西他滨协同抗癌作用的实验研究

The Synergetic Effect of Ginsenoside and Gemcitabine on Human Lung Cancer Cells Derived from Pleural Effusion

目的 研究人参皂甙 (GS)对癌性胸水中肿瘤浸润淋巴细胞 (TILs)增殖及抗肿瘤活性的影响 ,探讨活化后的TILs与吉西他滨 (GCM)的协同抗癌作用。方法 采用3 H -TdR释放法检测TILs的增殖及抗肿瘤活性 ,MTT比色法测定TILs与GCM的协同抗癌作用。结果 ( 1 )以GS处理TILs 72h ,其增殖速度明显高于PBS对照组 (P <0 .0 5 ) ,且GS浓度为 2～ 64μg mL时能增强IL - 2对TILs增殖的促进作用。 ( 2 )联合使用GS与白介素 - 2 (IL - 2 )能进一步提高TILs的抗肿瘤活性 ,对肺癌细胞的抑制率明显高于对照组 (P <0 .0 5 ) ,并且可减少IL - 2的剂量 ,其最适浓度为 2 3.7μg mL。 ( 3)GS和IL - 2合用后活化的TILs能提高GCM的细胞毒性 ,与单独使用GCM相比 ,P <0 .0 1。结论 GS是一种有应用前景的TILs诱导活化剂 ,在肿瘤免疫化疗中可能具有较重要的地位。

Objective:Our research aimed at studying whether GS can promote tumor infiltrating lymphocytes (TILs) derived from malignant pleural effusion proliferating and enhance their anti-tumor potency,elucidating the synergetic effect of TILs and gemcitabine (GCM).Methods:The proliferation and tumor cytotoxicity of TILs were determined by 3 H-TdR releasing assay.The synergetic effect of TILs and GCM was detected by MTT colorimetric assay.Results:(1)TILs treated with GS for 72 hours grew quicker than control groups(P<0.05),and GS can enhance the effect of IL-2 in accelerating TILs proliferation when its concentration is 2～64 μg/mL.(2)When used in combination with interleukin-2(IL-2),GS was capable of enhancing the tumor cytotoxicity of TILs in further,the inhibition rate to lung cancer cells was markedly higher than controls(P<0.05).On the other hand,GS can decrease the concerntration of IL-2 needed to show equivalent effect.The best concerntration of GS was 23.7 μg/mL.(3)TILs achivation by GS and IL-2 was capable of augmenting the tumor cytotoxicity of GCM (P<0.05).Conclusions: GS was a kind of antitumor agent which may be useed in clinical practice in future by inducing and activating TILs,thus it may play an important role in immunochemotherapy of malignant pleural effusion.