CyclinD1、C-erbB2及bcl-2基因在卵巢上皮性肿瘤中的表达及其临床意义

The expressions and clinical significances of CyclinD1,C-erbB2 and bcl-2 in ovarian epithelial tumors.

目的 :检测癌基因CyclinD1、C erbB2及bcl 2在卵巢上皮肿瘤中的表达 ,探讨它们在卵巢肿瘤发生、发展中的作用及临床、病理意义。方法 :应用免疫组化SP法检测72例恶性卵巢肿瘤、12例交界性卵巢肿瘤、2 1例良性肿瘤及 10例正常卵巢组织中Cy clinD1、C erbB2及bcl 2基因的表达情况。结果 :1.卵巢恶性、交界性及良性肿瘤中Cy clinD1阳性率依次为 2 7.78%、33.3%、9.5 2 %。恶性及交界性肿瘤阳性率明显高于良性肿瘤 ,其阳性率与组织学分级呈负相关 ,而与患者年龄、临床分期、组织学类型无关 ;2 .卵巢恶性、交界性及良性肿瘤中C erbB2的阳性率依次为 5 6 .9%、4 1.6 7%、14.2 8%。恶性及交界性肿瘤阳性率明显高于良性肿瘤 ,差异有显著性。C erbB2阳性表达在组织分化差及期别晚的肿瘤中较分化好、期别早者高 ;3.卵巢恶性、交界性、良性肿瘤中bcl 2的阳性表达率依次为 6 3.89%、5 0 %、2 8.5 %。恶性及交界性肿瘤与良性肿瘤之间的表达差异有显著性。组织分化差、期别早的肿瘤中bcl 2的阳性率较分化好、期别晚者高 ;4 .两种及两种以上基因同时表达率 (5 1.4 % )显著高于单基因表达 (2 7.79% )。CyclinD1与C erbB2基因表达呈负相关。结论 :CyclinD1、C erbB2及bcl 2基因在卵巢癌发生、发展中起重要作用 ,表明细胞?

Objectives:To study on the expression patterns of oncogenes CyclinD1,C-erbB2 and bcl-2 in ovarian epithelial tumors and to compare these patterns with clinicopathological features.Methods: SP methods was used to investigate the expressions of CyclinD1,C-erbB2 and bcl-2 genes in paraffin-embeded 72 samples of malignant ovarian tumors,12 samples of borderline ovarian tumors,21 samples of benign ovarian tumors and 10 samples of normal ovarian epithelial tissues. Results: 1.The positive rates of CyclinD1 in malignant,borderline and benign ovarian tumors were 27.78%,33.3% and 9.52% respectively.The expression rates in malignant and borderline cases were significantly higher than that in benign tumors.There was no significant difference between cyclinD1 expression and histologic type,age of patient as well as clinical stage,while there was a inverse significant difference between the histologic grades.2.The positive rate of C-erbB2 in malignant,borderline and benign ovarian tumors were 56.9%,41.67% and 14.28% respectively.The expression rate in malignant and borderline cases were significantly higher than that in benign tumors.The higher expression rate of C-erbB2 was found in those tumors which were more malignant,poorer differentiated and later stage.3.The positive rates of bcl-2 in malignant,borderline and benign ovarian tumors were 63.8%,50% and 28.5% respectively.The expression rates in malignant borderline cases were significantly higher than that in benign tumors.The expression rates were associated with differentiation of neoplasm tissues and clinical stage.The higher expression rates were found in those tumors which were poorer differentiated and earlier stage.4.The rate of the coexpressions of two or more oncogenes was significantly higher than that of one oncogene in ovarian tumor.Expression of cyclinD1 was negative relevant to C-cerbB2 expression in ovarian epithelial tumors. Conclusions: Expressions of oncogenes CyclinD1,C-erbB2 and bcl-2 in tissues of ovarian epithelial tumors are associated with clinicopathological features.They may play an important role in the development and progression of ovarian tumor.It further indicates that the disorder between cell proliferation and apoptosis plays an important role in tumorgenesis of ovarian tumor.Combined assay of C-erbB2,cyclinD1 and bcl-2 oncoprotein might be hopefully beneficial to early diagnosis,treatment and useful in evaluating the prognosis of ovarian tumors.