摘要
目的:明确蛋白激酶C和凋亡相关基因Bcl-2和Bax在大鼠嗅球中的表达分布及相关性,探讨蛋白激酶C(protein kinase C,PKC)对大鼠嗅球神经元凋亡的调节作用.方法:取青龄大鼠(4～6个月龄);成龄大鼠(10～12月龄);老龄大鼠(24～26月龄)各10只,断头处死,将大鼠嗅球置于4%的多聚甲醛中固定,连续切片,应用免疫组化方法检测PKC与凋亡基因Bcl-2和Bax在大鼠嗅球中的表达.结果:大鼠嗅球中的PKC表达老龄组(70.4±5.38)明显高于青龄组(20.44±1.21);而Bcl-2的表达青龄组(70.16±5.01)明显高于老龄组(24.10+4.00).差异显著(P＜0.01);且PKC和Bcl-2的表达呈负相关(r=0.8371).Bax随增龄逐渐增多,但差异不显著.结论:大鼠嗅球细胞的凋亡与PKC的调节密切相关;推测PKC对大鼠嗅球的生长、发育起着重要的负反馈调节作用.
ve: To determine the expression and distribution of protein kinase C (PKC) and apoptosis gene Bcl-2/Bax on olfactory bulb in rats. The relationship between protein kinase C and apoptosis gene Bcl-2/Bax were discussed. Methods: The experimental rats were divided into two groups, young group (10-12 months old) and senile group (24-26 months old). After removal of the heads of these rats, olfactory bulbs were immediately fixed with 4% paraformaldehyde, followed with paraffin-embedding, serial sectioning. The expressions of PKC, Bcl-2 and Bax proteins were determined by method of SP immunohistochemical staining. Results: The expression of PKC on the olfactory bulb markedly increased with aging, but the expression of Bcl-2 declined. The positive expression rate of PKC and Bcl-2 in young group was 20. 44±1. 21% and 70.16±5. 01% respectively; and in senile group was 70. 4 ±5. 38% and 24. 10±4. 00% respectively. Result showed that the expressions of PKC and Ble-2 had significant difference on young group and senile group ( P <0. 01). The relationship of expression of PKC and Bcl-2 was showed negatively relation. The expression of Bax gene gradually increased with aging, but there was no significant difference. Conclusion: PKC was closely related with apoptosis of the olfactory cells, it should be considered that negative feedback effect of PKC on the growth of olfactory bulb in rats.
出处
《耳鼻咽喉（头颈外科）》
2002年第2期113-115,W003,共4页
Chinese Arch Otolaryngology-Head Neck Surg
