摘要
Three thyroxine haptens were synthesized by protecting amino group of thyroxine with Cu 2+ ion and characterized by 1H NMR, MS, elemental analysis and IR. Thyroxine and the derivatives were coupled with carrier proteins(BSA and Ovalbumin). Thus antigens were prepared and hapten quantities of the antigens were measured by UV spectroscopy at 320 nm. An anti O methyl T 4 monoclonal antibody was prepared by immunization, cell fusion and screening.
Three thyroxine haptens were synthesized by protecting amino group of thyroxine with Cu 2+ ion and characterized by 1H NMR, MS, elemental analysis and IR. Thyroxine and the derivatives were coupled with carrier proteins(BSA and Ovalbumin). Thus antigens were prepared and hapten quantities of the antigens were measured by UV spectroscopy at 320 nm. An anti O methyl T 4 monoclonal antibody was prepared by immunization, cell fusion and screening.
出处
《应用化学》
CAS
CSCD
北大核心
2002年第4期387-389,共3页
Chinese Journal of Applied Chemistry
基金
中国科学院重大基金资助项目 (2 970 10 0 5 )
