摘要
应用多光子激发激光扫描显微镜对 5 羟色胺 (5 hydroxytryptamine ,5 HT)孵育的大鼠粘膜型肥大细胞进行自发荧光成像 ,首次观察到了活细胞内 5 HT相关的可见荧光 ,并对其产生机理进行了初步探讨 .实现了对活细胞内 5 HT空间分布的高分辨成像 ,为研究活组织或细胞内 5
The autofluorescence of 5-Hydroxytryptamine (5-HT) loaded rat mucosal mast cells (RBL-2H3 cells) was imaged with multi-photon excitation laser scanning microscope (MPELSM). Multi-photon excitated 5-HT relative visible fluorescence is observed in live cells for the first time, and the generating mechanism of 5-HT relative visible fluorescence is preliminarily studied. The spatial distribution of 5-HT in live cells was imaged at high spatial resolution in this present, which provides a new way to study the correlation between 5-HT spatial distribution and content, and the cellular functional state in live tissue or cells.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2002年第2期288-292,共5页
Progress In Biochemistry and Biophysics
基金
国家自然科学基金重点项目 ( 5 9836 2 40 )
国家杰出青年科学基金 ( 6 0 0 2 5 5 14 )
国家自然科学基金 ( 39870 2 0 5 )
高校博士点专项基金 ( 980 48733)~~
