哺乳动物精子质量的评价方法

Progresses on the Methods for Evaluating the Mammalian Sperm Quality

精子的各种功能状态反应了精子的受精能力。检测精子质膜完整性的荧光探针有SYBR 1 4、SY TO 1 7、CFDA、CDMFDA、CAM、PI、Hoechst 332 5 8、Hoechst3334 2 ,其中以SYBR 1 4结合PI使用效果最好。检测线粒体活性的荧光探针有JC 1、MITO、Rh1 2 3,JC 1比MITO和Rh1 2 3更适用于检测精子线粒体功能。检测顶体状态的荧光探针有PNA FITC、PSA FITC、LYSO G及CTC等。检测获能状态的荧光探针有CTC。此外 ,还可以通过检测精子与透明带的结合能力、精子穿入去透明带卵子的能力以及使卵子受精的能力和其后胚胎的发育能力等方面来评价精子的功能状态。

All aspects of functions in spermatozoon reflect its fertility. The fluorescent probes for assessing the integrity of sperm plasma membrane include SYBR-14, SYTO-17, carboxyfluorescein diacetate (CFDA), carboxy dimethyl fluorescein diacetate (CDMFDA), calcein acetyl methyl ester (CAM), propidium iodide (PI), Hoechst 33258, and Hoechst 33342, of which SYBR-14/PI is the best method. The fluorescent probes for determining sperm mitochondria activity include JC-1, Mitotracker green FM(MITO), and rhodamine123 (Rh123), of which JC-1 is better than MITO and Rh123. Acrosome integrity of spermatozoa can be examined with PNA-FITC, PSA-FITC, LYSO-G and chlortetracycline (CTC). CTC is also used to identify whether sperm is capacitated. Moreover, sperm quality can also be predicted through binding oocyte, penetrating the zona-free oocyte, and fertilizing oocytes.