摘要
采用反相液相色谱分离－脉冲安培检测和微透析取样技术,测定了取自鼠脑尾核和海马区微透析液中儿茶酚胺类神经递质的含量．对不同神经活动状态下取得微透析液中肾上腺素含量作了比较,将该法用于测定肾上腺素,灵敏度为２５μｇ/Ｌ．
A method for determination of catecholamine and related metabolite in the microdialysate fromrat brain by HPLC With pulsed amperometric detection was described. The optimum condition of chro-matographic separation and pulsed amperometri detection were studied. Sampling was carried out by mi-crodialysis technique from rat brain. A comparison of epinephrine(E) content in microdialysates from ratbrain at various event was made. The detection limit of the method is 25 μg/L for epinephrine.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2002年第4期552-554,共3页
Chemical Journal of Chinese Universities
基金
国家自然科学基金(批准号:29775027)
中国科学院基础性研究重大项目(批准号:KJ-951-A1-507-03)资助.
