抗核抗体对不同抗原的免疫应答

Antinuclear antibody can be induced by various antigens in mice

目的 探讨体内产生抗核抗体的抗原。方法 分别以羊红细胞、伤寒沙门菌及鸡血清免疫小鼠 ,同时设立生理盐水对照组 ,每组 10只 ,采用商品化酶联免疫法检测抗核抗体试剂盒 ,将酶结合物 (酶标抗人IgG)换成酶标金黄色葡萄球菌A蛋白 (SPA) ,建立检测抗核抗体的SPA ELISA法 ,并使其性能保持不变 ,用其检测小鼠的抗核抗体。结果 新建立的检测抗核抗体的SPA ELISA法与原法检测同一批标本 ,相关系数为 0 931,P <0 0 1。小鼠血清抗核抗体检测结果 (阳性率 ) :羊红细胞组为 90 % (9 10 ) ,伤寒沙门菌为 70 % (7 10 ) ,鸡血清组为 10 0 % (10 10 ) ,生理盐水对照组未检出抗核抗体。各实验组与对照组比较差异有显著性 (P <0 0 1)。结论 体内抗核抗体可能是一类由不同抗原刺激非特异产生的抗体。

Objective To explore the reason why antinuclear antibody (ANA) presents in serum.Methods Mice were immunized with sheep red blood cell (SRBC),Salmonella typhi and serum of chicken.The normal saline was injected to mice as control group.ANA in serum of mouse was detected with SPA ELISA,which was established by commerical ELISA kit to detect ANA,enzyme conjugate was changed into SPA labelled with enzyme instead of anti IgG labelled with enzyme.Results Same samples were examined with SPA ELISA and commercial ELISA kit,the correlation coefficient was 0 931, P <0 01.Positive rate of ANA was 90%(9/10),70%(7/10),100%(10/10) in SRBC,Salmonella typhi,serum of chicken,respectively,and was 0%(0/10) in control group.There was a significant difference compared test groups with control group ( P <0 01).Conclusion ANA may be antibodies induced by various antigens nonspecifically.