雌激素对去卵巢大鼠骨形态的影响

The effects of estrogen on bone histomorphometry in ovariectomized rats

目的观察大鼠去卵巢后椎体骨丢失的组织学改变、椎体骨密度和生物力学性能变化,了解激素复合药物对椎体骨丢失的影响。方法选用40只6月龄雌性SD大鼠,随机分为4组,每组10只,包括非手术组、假手术组、去卵巢组和去卵巢后药物治疗组。治疗组为去卵巢后3周给予盖福润(gevrine)0.8mg/kg,羟乙膦酸钠35mg/kg,1次/d。术后12周处死各组大鼠,进行骨形态计量学、骨密度及生物力学检测。结果与假手术组相比,去卵巢组的骨小梁体积占海绵骨体积百分比明显降低(P<0.01),椎体骨密度显著降低(P<0.01),骨的力学强度降低(P<0.05)。与去卵巢组相比,治疗组骨小梁体积占骨体积百分比明显增加(P<0.05),椎体骨密度明显增加(P<0.05),骨的力学强度增加(P<0.05)。结论雌激素复合药物盖福润和羟乙膦酸钠能抑制骨丢失,促进骨形成,对大鼠脊柱骨质疏松有明显的防治作用。

Objective To observe bone mineral density and biomechanical properties of lumbar vertebrae 12 weeks after ovariectomy in rats and to investigate the therapeutic effects of sex hormone on osteoporotic ovariectomized rat models.Methods Forty female Spraque Dawley rats at the age of six months were randomly divided into four groups(10 in each group): normal group, sham operation group, ovariectomized group and therapeutic group.Therapeutic group rats were fed for nine weeks with gevrine(0.8 mg/kg) and etidronate disodium(35 mg/kg) respectively every day.Double tetracycline labels were carried out on these animals before the sacrifice. All rats were killed 12 weeks after surgery. Bone mineral density was measured by dual energy X ray absorptiometry(DEXA).The right tibia was processed for methylmethacrylate embedding without decalcification and studied by bone histomorphometry.Results As compared with sham group, the percentage of volume (TBV/SBV) was significantly decreased in OVX rats(P< 0.01),bone mineral density was significantly decreased(P< 0.01), bone biomechanical properties were decreased(P< 0.05).However, after treatment with gevrine,TBV/SBV increased significantly as compared with that before treatment(P< 0.05), bone mineral density was significantly increased(P< 0.01), bone biomechanical properties were increased(P< 0.05).Conclusion Treatment with sex hormone could inhibit bone absorption stimulate bone formation. gevrine and etidronate disodium has some therapeutic effects on osteoporotic rat models.