氯化镧对内毒素诱导巨噬细胞肿瘤坏死因子表达的影响

The influence of lanthanum chloride on the TNFα expression of murine peritoneal macrophages stimulated by lipopolysaccharide

目的 探讨稀土化合物氯化镧对内毒素效应的影响。 方法 分离培养小鼠腹腔巨噬细胞 (M) ,用内毒素 (lipopolysaccharide ,LPS)对其进行诱导实验。采用ELISA法及SYBRGreen荧光定量RT PCR方法 ,观察氯化镧对LPS诱导的小鼠M肿瘤坏死因子α(TNFα)分泌及TNFαmRNA表达的影响。BALB/C小鼠随机分为 2组 ,分别给予致死量LPS及经氯化镧处理的LPS ,观察 7d内小鼠死亡率。 结果 经氯化镧处理的LPS诱导的小鼠 ,MTNFα分泌量及TNFαmRNA表达水平均明显低于LPS组 (P <0 .0 1)。经氯化镧处理的致死量LPS攻击小鼠 ,其死亡率明显低于LPS对照组。 结论 氯化镧具有降低内毒素毒性。

Objective To explore the influence o f lanthanum chloride on the TNFα expression of murine peritoneal macrophages stim ulated by lipopolysaccharide (LPS). Methods Murine peritoneal macrophages (M) were isolated , cult ured and then stimulated by LPS. The influence of lanthanum chloride on the TNF α secretion and TNFαmRNA expression of murine M stimulated by LPS was determ ined by ELISA method and SYBR green fluorescence quantitative RT PCR. Forty BAL B/C mice were randomly divided into two groups and were treated by lethal dose o f LPS and lanthanum chloride processed LPS, respectively. The mortality within 7 days was observed. Results The TNFα secretion and TNFαmRNA expression level of th e M from mice treated by lanthanum chloride processed LPS were obviously lower than those by LPS only (P<0.01). The mortality of the mice treated by letha l dose of LPS which has been processed by lanthanum chloride was significantly l ower than that by lethal dose of LPS only. Conclusion Lanthanum chloride possesed the capacity of lowering down the toxicity of LPS and inhibiting the TNFα secretion and TNFαmRNA expres sion in murine M stimulated by LPS .