肺癌患者血清与癌肿块中细胞因子的变化及其意义

A Prospective Study the Purpose and the Change of Cytokine in the Serum and the Tumor Block of the Lung Cancer Patients

目的:探讨肺癌患者血清与癌肿块中白介素-8(IL-8)、白介素-18(IL-18)、肿瘤坏死因子(TNFα)、血管细胞粘附因子-1(sVCAM-1)的变化情况。方法:采用Elisa方法检测12例正常对照,17例肺癌患者手术前后IL-8、IL-18、TNFα、sV-CAM-1在血清和癌旁肺组织、癌组织中的含量。结果:肺癌患者手术前后血清中的IL-8、IL-18、TNFα、sVCAM-1含量均比正常对照组增高有显著差异(P值均<0.01)。术后与术前两组比较术后TNFα含量有明显下降(P<0.05),其余3项指标有一定下降但无显著差异。癌肿块中IL-8、IL-18、TNFα、sVCAM-1含量比癌旁组织中的含量4项指标均显著增高(P<0.01,P<0.05)。结论:测定血清中的IL-8、IL-18、TNFα、sVCAM-1对肺癌的诊断及判断预后,鉴别良、恶性肿瘤有重要临床意义。

Objective: To research the change of interleukin-8(IL-8), interleukin-18(IL-18), tumor necrosis factor α (TNFα) and vascular cell adhesion molecule-1(sVC AM-1) in the serum and the tumor block of the lung cancer patients. Methods: 17 lung cancer patients was measured preoperatively and postoperatively with the amounts of IL-8,IL-18,TNFα and sVCAM-1 in the seram, the tumor block and the tissue around the tumor by Elisa method. Besides, 12 people' s semm was measured as controlled trial. Result: The preoperative and postoperative amounts of IL-8, IL-18,TNFαand sVCAM-1 in the seram of the lung cancer patients are significantly higher than the controlled trial (P < 0.01). The postoperative amount of TNFα obviously descended comparing with preoperative (P < 0.05) . The amounts of IL-8,IL-18 and sVCAM-1 descended, but the differences are not significantly. The amounts of IL-8, IL-18, TNFa andsVCAM-1 in tumor block are notable higher than in the tissue around the tumor (P < 0.01, P < 0.05) . Conclusion: Measuring the amounts of IL-8, IL18, TNFα and sVCAM-1 in the seram has important significance in the diagnosis, differential diagnosis and prognosis of lung cancer.