可溶性Fas与肠癌的相关性研究

Study on the relationship between serum soluble Fas and colorectal cancer

目的 (1)探讨结直肠癌患者血清中可溶性Fas的表达情况 ;(2)探讨结直肠癌的一些病理特征与可溶性Fas之间的相关性。方法 应用双抗体夹心ELISA法 ,对24例结直肠癌患者和24例正常对照组的血清中可溶性Fas浓度进行检测。结果 对照组血清中的Fas浓度平均值为(2.357±1.84)pg/ml。大肠癌无淋巴结转移组血清Fas平均值为(5.38±4.2602)pg/ml,大肠癌有淋巴结转移组Fas平均值为(7.114±3.065)pg/ml。大肠癌组血清中的sFas浓度明显高于正常对照组(P<0.05) ,有转移灶组大肠癌的sFas表达与无转移组无明显差异(P>0.05)。sFas的表达在大肠癌的不同分化程度和大小中无显著差异(P>0.05)。结论 (1)结直肠癌患者血清中的sFas明显高于正常对照组 ;(2)血清中的可溶性Fas浓度与大肠癌有无淋巴结转移和癌细胞的分化程度及癌肿的大小无明显相关性。

Objective (1)To explore the expression of serum soluble Fas in patients with colorectal cancer. (2) To investigate the relationship between the pathologic character of colorectal cancer and serum soluble Fas . Methods A double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) was used to detect the serum soluble Fas level in 24 patients with colorectal cancer and 24 normal control people. Results The average serum Fas concentration was (2.357±1.84)pg/ml in control group, (5.38±4.2602)pg/ml in patients without lymph node metastasis ,and (7.114±3.065)pg/ml in cases with lymph node metastasis , respectively. The serum Fas level in patients with colorectal cancer was significantly higher than that in control group (P<0.05), but there was no significant difference between the patients with and without lymph node metastasis (P>0.05) .The expression of serum Fas had no significant difference among patients with different differentiation and size of the tumor (P>0.05). Conclusions (1) The serum Fas level in patients with colorectal cancer is significantly higher than that in control group; (2) There is no significant correlation between the serum Fas expression and lymph node metastasis, differentiation and size of colorectal cancer.