HCG的生物电化学免疫分析

BIOELECTROCHEMICAL IMMUNOASSAY OF HUMAN CHORIONIC GONADOTROPIN

共价耦联HCG抗体的醋酸纤维素膜,当表面发生抗体与抗原复合的免疲化学反应后、膜的电荷密度向负值减小的方向移动,膜电位随抗原浓度的增大,向正方向移动。根据膜电位与抗原浓度之间的关系,可构成测定HCG的膜电位免疫传感器。利用夹心原理,在膜表面形成抗体-抗原-酶标抗体的夹心结构所组成的酶免疫传感器,具有很高的检测灵敏度,且与放射免疫测定显著相关。

The antibody to HCG was immobilized covalently to the cellulose acetate membrane. The charge density of membrane shifted to a decrease of negative charge when the immunochemical reaction occurred on the membrane surface. The membrane potentials shifted to positive values with increase of HCG concentrations. The membrane potential immunosensor for the measurement HCG was constructed according to the relationship of the membrane potentials and the HCG concentrations. The enzyme immunosensor for HCG by use of the sandwich principle was discussed. The biosensor was applied to the determination of HCG in the concentration range of 5 to 2500 mlU/ml and gave a good correlation with radioimmunoassay.