利用生物信息学鉴定新发现的膜联蛋白亚家族anx32

Application of bioinformatics analysis in identifying a novel annexin subfamily-anx32

目的：对抗原基因cC1进行结构分析和初步功能研究。方法：利用网上生物信息学软件进行cC1的一级结构、二级结构分析，同源建模预测三级结构。白陶土部分凝血活酶时间（KPTT）检测重组蛋白GST－anx32的抗凝血活性。结果：cC1在核酸水平和氨基酸水平均与膜联蛋白基因同源，具有4个同源结构区域，且每一个同源区域均具有膜联蛋白的典型motif“G－X－G－T（38 residues）－D／E”。抗凝血实验表明大肠杆菌表达的谷胱甘肽转移酶－cC1融合蛋白具有很强的抗凝血活性。结论；抗原基因cC1具有膜联蛋白家族的典型特征，但与已知的31个亚家族氨基酸序列的同源性均低于48％，因此是一个新的膜联蛋白亚家族成员，命名为膜联蛋白32（anx32）。为进一步研究其生理功能及核酸疫苗pcDNA3－γcC1诱导囊尾蚴细胞凋亡的分子机制打下了基础。

Objective: To investigate the structural characteristics and the primary functions of antigen gene cC1. Meth-ods: The primary and the secondary structures were analyzed using bioinformatics programs provided by Internet servers.The predicted 3D structure of cC1 was established by homology protein modeling method. Anticoagulant activity of GST-anx32 was assayed by modified kaolin partial thromboplastin time (KPTT). Results: cC1 had high homology to annexinsgenes both at nucleic acid and at amino acid level. It contained 4 homologous regions, and each region included the typical an-nexin motif 'G-X-G-T (38 residues)-D/E'. The results of KPTT assay showed that the recombinant protein GST-anx32 hadhigh anticoagulant activity. Conclusion: cC1 has the common structures of annexins but the homology to the extant annexinsis no more than 48%, cC1 is a member of a novel annexin subfamily and designed as annexin32.