摘要
烟草花药培养获得单倍体植株 ,进而诱导产生单倍体愈伤组织 ,用硫酸二乙酯 (DES)进行诱变处理 ,其半致死浓度为 0 .32 %。处理后的单倍体愈伤组织接种到含有 15 %聚乙二醇 (PEG)的筛选培养基上 ,PEG耐受突变体诱导率为 9.8% ,突变体与对照相对电导率和丙二醛 (MDA)
Haploidic seedlings of tobacco(Nicotiana tabacum L.) were obtained by anther culture and then haploidic callus were initiated. Half death concentration of diethylsulfate (DES) treatment for mutant inducement is 0.32%. When the treated haploidic callus inoculated on the selection medium contains 15% PEG, 9.8% PEG resistant mutants were induced. Difference of the relative electrical conductivity and the content of malondialdehyde (MDA) between mutants and control reached very significant level.
出处
《贵州农业科学》
CAS
2002年第2期5-7,共3页
Guizhou Agricultural Sciences
