实验性免疫应激对雏鸡血液激素与免疫功能的影响

Effects of Experimental Immunologic Stress on Blood Hormone and Immunity in Chickens

将 2 0 0只 2周龄雏鸡随机分成试验组 (T)和对照组 (C)。以鸡新城疫 (ND)Ⅰ系疫苗作为免疫应激原 ,给T组雏鸡肌肉接种 2头份 /(mL·只 ) ,诱发免疫应激 ;C组不做任何处理。分别于接种后不同时间检测免疫应激对试验鸡血液激素水平和免疫功能的影响。结果 ,T组雏鸡血液中促肾上腺皮质激素 (ACTH)、皮质酮 (Cor)、甲状腺素 (T4 )显著 (P <0 0 5 )或极显著 (P <0 0 1)高于C组 ,血液中T淋巴细胞对PHA的反应性显著降低 ,血清总IgG先减少 ,后增多。血液中皮质酮与T淋巴细胞转化试验结果呈负相关、与血清IgG变化无相关性。提示发生免疫应激后 ,鸡血液中ACTH ,Cor ,T4 会发生明显变化 ;体液免疫功能先受到抑制 ,然后得到加强 ;

two week old chickens were randomly divided into treatment group(T) and control group(C) in this experiment.Newcastle Disease Ⅰ Series(NDⅠ) was taken as the source of immunological stress;the muscles of chicken group T were inoculated with 2 dose per chicken to induce immunological stress,and group C wasn′t given any treatment.The effects of immunological stress on blood hormone level and immunity function of experimental chickens were detected at different time after inoculation.The results shows that content of ACTH、Cor and T 4 in the blood of group T were higher(p<0 05) or significantly higher(p<0 01)than that of group C;The reaction properties of T lymphocyte in blood to PHA decreased greatly,the total content of IgG in serum first decreased and later increased.There is negative correlationship between the content of Cor in blood and the transformation rate of T lymphocyte,and there isn′t any correlationship between the content of Cor in blood and the change of IgG in serum.Result indicated that,after the occurrence of immunological stress,ACTH、Cor and T 4 in chicken blood will change evidently,the immunological function of humoral was first restrained,then inhanced;the immunological function of cell was restrained,too.