玫瑰、月季、蔷薇等蔷薇属植物RAPD分析

The RAPD analysis of the Rosa genus Plant of R.rugosa , R.chinensis and R.davurica

对蔷薇属的玫瑰、月季、蔷薇 3个种的 15个品种进行RAPD分析 ,16个引物对受试品种PCR扩增共获得 2 0 2条谱带 ,其中 12 3条 (6 0 .9% )表现多态性。利用UPGAMA法构建分子系统进化树 ,以相似性系数 0 .5为阈值 ,玫瑰与月季为一个聚类组 ,蔷薇为另一个聚类组 ;以相似性系数 0 .6为阈值 ,4个蔷薇品种分为 4个不同的聚类组 ,5个月季品种为一个聚类组 ,5个玫瑰品种为一个聚类组 ,平阴紫枝玫瑰单独为一个聚类组 ,表明玫瑰与月季的亲缘关系较近 。

Rosa rugosa , R.chinensis and R.davurica are belonged to Rosa genus , 15 cultivars were amplified by 16 primers, which produced 202 bands and 123 polymorphism bands. And the genetic tree was constructed by the ways of UPGMA method. It confirmed that R.rugosa and R.chinensis belonged to the one cluster group and R.davurica belonged to another by the dissimilarity of0.5. Moreover, dissimilarity of0.65 could resulted in 4 R.davurica to be divided into 4 cluster groups, respectively. While 5 R.chinensis belonged to one group. Meanwhile, 5 R.rugosa were belonged to one group, and 1 R.rugosa was one independent group. Therefore, the genetic relationship was close between R.chinensis and R.rugosa , and they were distant to R.davurica in genetic relationship.