二甲基亚砜抑制胶原蛋白形成的机制

Inhibitory effect of dimethyl sulfoxide on collagen formation

目的 从分子水平探讨二甲基亚砜 (DMSO)抑制组织扩张器周围纤维囊增生的机制。方法 以大白鼠为动物模型 ,在其背部埋置扩张器后 ,注入 30 %的DMSO为用药组 ,生理盐水为对照组。扩张皮肤软组织后 ,切取囊壁 ,利用原位杂交和免疫组织化学技术 ,从分子水平测定两组扩张器周围纤维包囊囊壁中Ⅰ、Ⅲ型胶原含量和Ⅰ、Ⅲ型前胶原mRNA的表达量。结果 用药组周围纤维包囊囊壁中Ⅰ、Ⅲ型胶原含量和Ⅰ、Ⅲ型前胶原mRNA的表达量均小于对照组。结论 DMSO对纤维包囊囊壁中Ⅰ、Ⅲ型胶原的抑制 ,可能是通过抑制成纤维细胞的Ⅰ。

Objective To explore the mechanism of inhibitory effect of dimethyl sulfoxide (DMSO) on the hyperplasia of the fibrous capsule around the tissue expander. Methods The experimental model was established in rats as follows: after implanting the expander on the back of rats, 30 % DMSO or normal saline was injected into the expander. The former was classified as experimental group, and the latter as control group. The cystic wall was resected after the skin and soft tissues were expanded. In situ hybridization and the immunohistochemical staining were used to determine the expression of typesⅠand Ⅲ of collagen and procollagen mRNA in the cystic walls. Results It was found that both the typeⅠand Ⅲ of collagen content and the expressing quantity of mRNA of procollagen in the cystic wall of the experimental group were less than those of control group. Conclusion The results imply that the mechanism that DMSO inhibits the expression of typesⅠand Ⅲ of collagen in the fibrous cystic wall may be achieved through down-regulating the genetic expression of procollagen in the fibroblasts.