摘要
将 Ti O2 、粘土、Si O2 、Ca CO3 及活性炭混合 ,经高温烧结 ,制得可用于柱层析的 Ti O2 多孔陶瓷。以陶瓷柱层析结合超滤法纯化果菠萝蛋白酶 ,产品比活力 937u/ mg蛋白 ,活力回收率 48% ,纯化倍数
The porous TiO 2-pottery was prepared by calcining TiO 2 powder together with clay, CaCO 3, SiO 2 and activated charcoal, and could be used as chromatographic separation material. The column chromatography combined with ultrafiltration was applied to the purification of fruit bromelain. The results showed that the specific activity of the purified bromelain was 937 u/mg protein with activity recovery of 48% and the purification multiple was 8.0.
出处
《中国医药工业杂志》
CAS
CSCD
北大核心
2002年第3期121-123,共3页
Chinese Journal of Pharmaceuticals
关键词
果菠萝蛋白酶
TiO2多孔陶瓷
超滤
纯化
fruit bromelain
porous TiO 2-pottery
ultrafiltration
purification
