孕妇血清与胎儿脐血HBV-DNA定量检测及其相关性研究

Quantitative Examine and Correlation Analysis on Pregnant Women's Serum and Fetal's Cord Blood HBV-DNA

为了探讨母婴之间 HBV- DNA含量的关系 ,应用 Amplisensor荧光定量 PCR方法检测 79例 HBV感染孕妇血清及胎儿脐血 HBV- DNA含量。结果 :2 6例 HBe Ag (+)和 5 3例 HBe Ag (- )孕妇其静脉血、脐血 HBV- DNA阳性率分别为 10 0 .0 %、 85 .0 %和 6 2 .3%、 35 .8% ,其含量 (对数值 )分别为 7.780± 0 .82 3、 5 .12 2± 0 .883和 5 .46 0±1.375、 3.96 1± 0 .912 (P均 <0 .0 0 5 ) ,其中 42例脐血 HBV- DNA阳性者其母血也呈阳性 ;孕妇血清与胎儿脐血 HBV-DNA的含量呈线形相关 (r=0 .2 70 ,P<0 .0 5 )。提示 :孕妇血清 HBV- DNA含量的高低反映 HBV垂直感染的强弱。

To study the relation between the load of serum HBV-DNA in pregnant women infected with HBV and intrauterine infection of their newborns,serum HBV-DNA was determined by quantitatine polymerase chain reaction in 79 pregnant women infected with HBV and their newborns' unbilical cord blood.Results, positive rate of serum HBV-DNA in pregnant with HBeAg positive or HBeAg negative and their fetal umbilical blood is 100%,85%and 62.3%,35.8%,respectively and the value of logarithm of HBV-DNA copies is respectively 7.780±0.823,5.122±0.883 and 5.460±1.375,3.961±0.912.HBV-DNA in 42 samples of fatal umbilical blood is positive along with their mother's serum HBV-DNA positive.The level of HBV-DNA in fetal umbilical blood is positive relative to serum HBV-DNA load in pregnant women.Serum HBV-DNA load in HBV carrying pregnant women decides the level of HBV-DNA in their fetus.