加味茯苓甘草汤防治慢性常压缺氧性肺动脉高压的实验研究

Experimental Studies of Modified Filling Gancao Decoction in Preventing and Treating Chronic Normotensive Hypoxic Pulmonary Hypertension

[目的]探讨中药复方制剂加味茯苓甘草汤(茯苓、甘草、桂枝、桃仁、苇茎等组成)对慢性肺动脉高压(ＨＰＨ)的防治作用。[方法]常压缺氧法建立ＨＰＨ大鼠模型,以生理盐水为对照药,设对照组、缺氧组和中药治疗组,灌胃３周,检测平均肺动脉压力(ｍＰＡＰ),观察右心室与左心空加室间隔重量之比[ｍＲ/(ｍＬ＋ｍＳ)]、血浆及肺匀浆中磷脂酶与Ａ２(ＰＬＡ２)、血栓素Ｂ２(ＴＸＢ２)、６－酮－前列腺素(６－ｋｅｔｏ－ＰＧＦ１α)、超氧化物歧化酶(ＳＯＤ)及丙二醛(ＭＤＡ)的变化。[结果]①缺氧组大鼠ｍＰＡＰ、ｍＲ/(ｍＬ＋ｍＳ)、ＰＬＡ２、ＴＸＢ２、ＭＤＡ含量增加,６－ｋｅｔｏ－ＰＧＦ１α、ＳＯＤ含量下降,与对照组相比有显著性差异(Ｐ＜０．０１);③加味茯苓甘草汤能降低ｍＰＡＰ、ｍＲ/(ｍＬ＋ｍＳ),与缺氧组相比,前者有显著性差异(Ｐ＜０．０１);③加味茯苓甘草汤能使肺组织中ＰＬＡ２、６－ｋｅｔｏ－ＰＧＦ１α、ＴＸＢ２、ＭＤＡ含量降至正常水平,与缺氧组相比差异有显著性(Ｐ＜０．０１或Ｐ＜０．０５);④加味茯苓甘草汤能使血浆中ＰＬＡ２、６－ｋｅｔｏ－ＰＧＦ１α、ＳＯＤ含量下降,与缺氧组相比差异有显著性(Ｐ＜０．０１或Ｐ＜０．０１),ＴＸＢ２、ＭＤＡ含量无明显变化。[结论]长期使用加味茯?

[Objective] To investigate the preventive and therapeutic effect of Modified Filling Gancao Decoction (MFGD) for chronic normotensive hypoxic pulmonary hypertension. [Methods] Twenty - nine rats were allocated to three groups: normal control group (Group A), model group ( Group B) and MFGD group ( Group C). After three -week gastric infusion of normal saline or MFGD, mean pulmonary arterial pressure(MPAP), right ventricle and left ventricle added with interventricular septum weight ratio[ mR/( mL + mS) ] and the levels of phospholipase A2(PLA2),thromboxane B2 (TXB2), 6- keto - PGF1a, superoxide dismutase (SOD) and malonaldehyde (MDA) in plasma and lung homogenate were measured. [Results] MPAP, mR/ (mL+ mS) and the levels of PLA2, TXB2 and MDA increased and 6 - keto - PGF1a and SOD levels decreased in Group B (P<0.01as compared with Group A). In Group C, MPAP, mR/ (mL+mS) and the levels of PLA2 and 6 - keto - PGF1a in plasma and lung homogenate were decreased, TXB2 and MDA in lung homogenate and SOD in plasma were also decreased (P <0.01 or 0.05as compared with Group B). [ Conclusion ] Long - term medication of MFGD can prevent and treat chronic hypoxic pulmonary hypertension, and its mechanism may be related to the inhibition of PLA2 and associated inflammatory medium and the reduction of the toxicity of free radicals.