黄连与吴茱萸不同比例组成的方剂的不同药理作用研究

Effect of Combination of Rhizoma Coptidis and Fructus Evodiae in Various Proportions on Rat Models with Heat Syndrome Induced by Radix Codonopsis Pilosulae and Radix Astragali seu Hedysari

【目的】探讨黄连与吴茱萸不同比例组成的方剂的不同药理作用。【方法】用党参与黄芪给大鼠灌胃造成类热证模型,观察不同比例配伍的黄连与吴茱萸对它们的影响。【结果】黄连与吴茱萸比例为６:１的左金丸和２:１的甘露散有显著的消除类热证的作用,ｌ:ｌ的茱萸丸对肝细胞超微结构的影响中表现出有减轻类热证的作用,而体温(ｔ)、血清皮质醇(Ｃｏｒ)、１７－羟皮质类固醇(１７－ＯＨＣＳ)的改变则未见减轻类热证或增强类热证的作用。【结论】认为不同比例配伍的黄连与吴茱萸对类热证的病理改变有不同的影响。

[Objective] To observe the effect of combination of Rhizoma Coptidis (RC) and Fructus Evodiae (FE) in various proportions on rat models with heat syndrome. [Methods] Rat models with heat syndrome were set up by gastric infusion of Radix Codonopsis Pilosulae and Radix Astragali seu Hedysari. Fifty rats were allocated to five groups: normal control group (Group A), model group (Group B), model rats treated with RC and FE at the proportion of 6∶1 (Group C), model rats treated with RC and FE at the proportion of 2∶1 (Group D) and model rats treated with RC and FE at the proportion of 1∶1 (Group E). [Results] Ultrastructure of hepatic cells were improved in Group C, Group D and Group E. Indexes of heat syndrome such as temperature, serum corticosteroid (Cor) level and 17 - hydroxycorticosteroid (17-OHCS) content in homogenate of adrenal glands were decreased in Group C and Group D but stayed unchanged in Group E as compared with Group B ( P < 0.05 or 0.01). [ Conclusion] Combination of RC and FE in various proportions exerts different effects on rat models with heat syndrome induced by Radix Codonopsis Pilosulae and Radix Astragali seu.