EGF对肝部分切除术后促进肝再生的实验研究

EPIDERMAL GROWTH FACTOR PROMOTED HEPATOCYTE REGENERATION AFTER PARTIAL HEPATECTOMY IN RATS

目的 寻找能够促进肝细胞再生的药物。方法 采用形态计量技术 ,3 H -TdR活体渗入实验以及动脉血中酮体比率的变化来研究上皮生长因子 (EGF)对大鼠部分肝切除术后残肝能量代谢、DNA合成、肝细胞有丝分裂以及肝再生的变化。结果 肝细胞核分裂指数、肝细胞核体积密度、新生肝细胞数目密度、酮体比率及3 H -TdR活性渗入实验结果均显著高于对照组 (P <0 .0 5或P <0 .0 1)。结论 EGF对大鼠肝部分切除术后残肝有促进其再生作用。

OBJECTIVE To study the drug that promots the hepatocyte regeneration after partial hepatectomy in rats.METHODS Quantitative morphometry technique, 3 H-TdR in vivo test and arterial ketone body ratio (AKBR) were employed to evaluate the energy metabolism, DNA synthesis, and liver cell nuclear mitosis an hepatocyte regeneration of the residual liver tissue in rats after partial lobectomy when treated with epidermal ghowth factor(EGF).RESULTS After partial hepatectomy in the experimental group, liver cell nuclear mitosis, nuclear density, new liver cell numbers, AKBR and 3 H-TdR in vivo test were much higher than those in the control group (P<0.01 or P<0.05).CONCLUSION EGF promotes the hepatocyte regeneration of the residual liver.