癌宁诱导人胃腺癌细胞SGC-7901凋亡的形态学变化及未端标记

Apoptotic Morphological Changes and TUNEL of Human Gastric Cancer Cell SGC-7901 Induced by Aining

目的:探索癌宁对人胃癌细胞的诱导凋亡作用。方法:采用体外细胞培养技术,通过形态学、分子生物学方法,对中药复方癌宁和顺铂诱导胃癌细胞凋亡进行了深入研究。结果:经癌宁及顺铂处理后的细胞出现凋亡形态学改变;癌宁在1 g/L浓度对细胞的凋亡诱导率是46.36％,与25 mg/L顺铂对细胞的凋亡诱导率49.12％间无显著差异(P>0.05),两者均与空白对照组有显著差异(P<0.01)。结论:癌宁及顺铂对人胃癌细胞具有诱导凋亡作用,中药复方癌宁可望成为一种有前途的新的癌细胞凋亡诱导剂。

Objective: To investigate the inducing apoptosis effect of Aining on the human gastric cancer cell SGC-7901. Methods: In vitro cell culture was adopted to explore the inducing apoptosis efiect of Aining and cisplation (DDP) through the morphological method and the molecular method.Results: Apoptotic morphological changes were seen after the cells cultured with Aining or DDP;apoptosis rate of 1 g/L Aining group was 46.36%, which had no significant differences with the apoptosis rate 49.12% of the 25 mg/L DDP group; both of the Aining and the DDP groups were significantly different from that of the blank group (P < 0.01). Conclusions: Both DDP and Aining could induce the apoptosis of human gastric cancer cell and the traditional medicine compound prescription Aining is likely to become a new agent which is used to induce apoptosis of cancer cells.